SKRINING BAKTERI DARI AIR SUNGAI KARANG MUMUS SEBAGAI PENGHASIL ENZIM PROTEASE, SELULASE DAN LIPASE

Abstract

Bacteria from polluted waters have the potential to produce extracellular hydrolase enzymes, namely protease enzymes, cellulase and lipase. This study was conducted to isolate bacteria from the water of the Karang Mumus River around Segiri Market, Samarinda as a producer of hydrolase enzymes. The bacteria were isolated in a nutrient-dense agar (NA) medium by the streak method. The results of bacterial isolation were obtained from 14 single colonies of bacteria. Single colonies of bacteria are selected to produce hydrolase enzymes in the form of proteases, cellulases and lipases. The results of the selection of hydrolase enzyme-producing bacteria were 3 protease-producing isolates, 10 cellulase-producing isolates, and 5 lipase-producing isolates.