SCREENING AMILASE PRODUCING BACTERIA FROM DONDANG HOT SPRINGS SEDIMENT MUARA JAWA

  • M Nur Yasin Fahrul Fhozi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
  • Winni Astuti Jurusan Kimia FMIPA Universitas Mulawarman
  • Rahmat Gunawan Jurusan Kimia FMIPA Universitas Mulawarman

Abstract

The screening of amylase enzyme producing thermophilic bacteria from sediment of hot spring in Muara Jawa has been done. Screening has done by using selective media of starch, will show the formation of clear zones around the colonies by dripping the iodine solution. From alot colonies selected 1 colony with  widest clear zone. Single colony obtained was identified as Bacillus sp. Amylase produced was tested for amylase activity by DNS method and obtained the amylase activity at pH 6 and temperature at 55°C is 2,8460 U/mL.

References

[1] Sianturi, D. C. 2008. Isolasi Bakteri dan Uji Aktivitas Amilase Termofil Kasar dari Sumber Air Panas Penen Sibirubiru Sumatera Utara. Medan.
[2] Octarya. 2010. Purifikasi Parsial Enzim Ektraseluler yang Diisolasi dari Sumber Air Panas Bukit Kili Solok sert aplikasinya untuk Menghidrolisis Limbah Berserat. Jurnal Natur Indonesia, 106–114.
[3] Alariya, S. S. 2013. Amylase Activity Of A Starchdegrading Bacteria Isolated From Soil. Arch Appl Aci Res, 5, 15–24.
[4] Prakash, O. 2010. Alpha-Amylase: An Ideal Representative Of Thermostable Enzyme. Appl Ication Biochem Biotechnology, 4, 2124
[5] Bisset, A. 2007. Bacterial Community Shift in Organically Pertubed Sediment. Environmental Microbiology, 9, 46–60.
[6] Palmer, T. 1985. Understanding Enzyme. New York: Wiley and Sons Publisher.
[7] Miller, G. L. 1959. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry, 31, 426–428.
[8] Apriyantono, A., Fardiaz, D., Puspitasari, N. L., Sedarnawati, & Budiyanto, S. 1988. Analisa Pangan. Bogor: PAU Pangan dan Gizi IPB.
Published
2017-11-29
How to Cite
FAHRUL FHOZI, M Nur Yasin; ASTUTI, Winni; GUNAWAN, Rahmat. SCREENING AMILASE PRODUCING BACTERIA FROM DONDANG HOT SPRINGS SEDIMENT MUARA JAWA. JURNAL ATOMIK, [S.l.], v. 2, n. 2, p. 213-215, nov. 2017. ISSN 2549-0052. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/487>. Date accessed: 14 dec. 2017.
Section
Artikel