WAKTU PRODUKSI OPTIMUM LIPASE DARI BAKTERI ENDOFIT DAUN Macaranga hullettii King ex Hook.f.
Abstract
Endophyte bacteria isolate from the leaves of Macaranga hullettii King ex Hook.f. has potential to producer lipase. In the study was conducted to filter lipase producing endophyte bacteria from Macaranga hullettii King ex Hook.f. and determine the optimum production time. The results of screening endophyte bacteria showed 6 isolates of endophyte bacteria that had the potential to produce lipase and 1 isolate was chosen which is number 5 to determine the optimum production time. The optimum production time of lipase from Macaranga hullettii King ex Hook.f. leaves for 72 hours with activity that is 2.92 U/mL.
Keyword
Endophyte Bacteria, Macaranga hullettii King ex Hook.f., Lipase, Optimum Production Time
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References
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Barauskas, R. A. 1984. Lipid-Lipase Interaction
I. Fat Splitting With Lipase from Candida
Rogusa. JAOCS, 61(6), 1067-1071.
[2] Susanty, A., Fitriani, Candra, K. P. 2013. Isolasi
Bakteri Penghasil Lipase Dengan Kemampuan
Esterase Untuk Produksi Metil Ester. J Riset
Tech Ind, 134-142.
[3] Radji, M. 2005. Peranan Bioteknologi dan
Mikroba Endofit Dalam Pengembangan Obat
Herbal. Majalah Ilmu Kefarmasian Vol. 2 No. 3,
113-126.
[4] Djafar, F., Purwandaria, T., Sinurat, A. P. 2010.
Isolation of Endophytic Bacteria from Palm Oil
Fruits and Characterization of Their Lipases.
Microbiol Vol. 4 No. 2, p 1-6.
[5] El-Deeb, B., Fayez, K., Gherbawy, Y. 2013.
Isolation and Characterization of Endophytic
Bacteria from Plectranthus tenuiflorus
Medicinal Plant in Saudi Arabia Desert and
Their Antimicrobial Activities. J Plant
Interactions Vol. 8 No. 1,56-64.
[6] Pramiadi, D., Yulianti, E., Rakhmawati, A.
2014. Isolasi Dan Uji Aktivitas Enzim Lipase
Termostabil Dari Bakteri Termofilik Pasca
Erupsi Merapi. J Sains Dasar, 3(1), 9-19.
[7] Kouker, G dan Jaeger K. E. 1986. Spesific and
Sensitive Plate Assay for Bacterial Lipases.
Applied and Environmental Microbiology, 211-
213.
[8] Dwidjoseputro, D. 1998. Dasar-dasar
Mikrobiologi. Jakarta: Djambatan.
[9] Andersson, R. E. 1980. Production Lipolysis and
Formation of Volatile Compounds by
Pseudomonas fluoroscens in Fat Containing
Media Junk Food. Microb Technol Vol. 45:
1694-1701.
[10] Tripanji, Suharyanto, Arini, N. 2008. Lipase
Spesifik 1,3-Gliserida Dari Fungi Lokal Untuk
Biokonversi CPO Menjadi Diasilgliserol.
Menara Perkebunan, 76(1), 11-22.
Published
2019-03-30
How to Cite
MARLIANI, Nelly; ASTUTI, Winni; KARTIKA, Rudi.
WAKTU PRODUKSI OPTIMUM LIPASE DARI BAKTERI ENDOFIT DAUN Macaranga hullettii King ex Hook.f..
JURNAL ATOMIK, [S.l.], v. 4, n. 1, p. 6-8, mar. 2019.
ISSN 2549-0052.
Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/636>. Date accessed: 26 apr. 2024.
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