PENGGUNAAN SPEKTROFOTOMETER UV DAN HPLC PADA ANALISIS KANDUNGAN KAFEIN KOPI ARABIKA DAN ROBUSTA

  • Dedy Prasetyo Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Saibun Sitorus Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Rahmat Gunawan Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University

Abstract

Analysis of caffeine content in Arabica coffee and Robusta coffee using Ultra Violet (UV) spectrophotometers and High Performance Liquid Chromatography (HPLC) was carried out. Coffee samples were soxhleted with 96% ethanol with the rotation variation used, namely 4, 6, 8, 10 and 12. The extract was dissolved in 96% ethanol and tested with a UV spectrophotometer. The results obtained  caffeine content in arabica coffee for 4, 6, 8, 10 and 12 rotations are 1.347 mg/L, 1.631 mg/L, 1.953 mg/L, 2.868 mg/L and 3.764 mg/L respectively. While the caffeine content obtained by Robusta coffee for 4, 6, 8, 10 and 12 turns are 1.820 mg/L, 2.258 mg/L, 2.741 mg/L, 3.814 mg/L and 4.781 mg/L, respectively. Caffeine content in Arabica and Robusta coffee were analyzed by HPLC. The results obtained in Arabica coffee are retention time of 5.079 minutes, area of 1265348 and height of 56539 and in Robusta coffee the retention time is 5,083 minutes, the area is 1249273 and the height is 55700.

Keyword
Arabica Coffee, Robusta Coffee, 96% ethanol, Soxhletation, Caffeine, UV Spectrophotometer

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Published
2021-01-06
How to Cite
PRASETYO, Dedy; SITORUS, Saibun; GUNAWAN, Rahmat. PENGGUNAAN SPEKTROFOTOMETER UV DAN HPLC PADA ANALISIS KANDUNGAN KAFEIN KOPI ARABIKA DAN ROBUSTA. JURNAL ATOMIK, [S.l.], v. 5, n. 2, p. 76-80, jan. 2021. ISSN 2549-0052. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/734>. Date accessed: 16 jan. 2021.
Section
Artikel