UJI FITOKIMIA DAN TOKSISITAS (Brine Shrimp Lethality Test) EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN FRAKSI ETANOL SISA DARI DAUN AJERAN (Bidens pilosa L.)
Abstract
Phytochemical test and toxicity in ethanol extract, ethyl acetate fraction and residual ethanol fraction of ajeran leaves (Bidens pilosa L.) has been done. The method used to determine secondary metabolites is clualitative test and the value of Lethal Concentration (LC50) with the Brine Shrimp Lethality Test (BSLT). Phytochemical test results showed that the ethanol extract and ethyl acetate fraction contained flavonoid, steroid and phenol compound. Residual ethanol fraction contained flavonoid and phenol compound. Toxicity test showed ethanol extract had LC50 value of 862.19 ppm. Ethyl acetate fraction has LC50 value of 8.26 ppm. Residual ethanol fraction had LC50 value of 275.24 ppm. The results of the toxicity test can be concluded that the ethyl acetate fraction very toxic category, ethanol extract and residual ethanol fraction included toxic category. The results that obtained from GC-MS characterization were steroid
Downloads
References
[2] Bartolome A. P. Villasenor I. M. dan Yang W. 2013. Bidens pilosa L (Asteraceae): Botanical Properties. Traditional Uses, Phytochemisrty, and Pharmacology. Evidence-Based Complementary and Alternative Medicice. 2: 1-3.
[3] Ariyanti N. K. 2007. Uji Aktivitas Antioksidan Fraksi air ekstrak metanolik herba ketul (Bidens pilosa L.). Skripsi. Yogyakarta: Universitas Gajah Mada.
[4] Nursarini N. M. R. 2007. Uji aktivitas antioksidan fraksi etil asetat ekstrak metanolik herba ketul (Bidens Pilosa L.). Skripsi. Yogyakarta: UGM.
[5] Darwis D. 2000. Teknik dasar laboratorium dalam penelitian senyawa bahan alam hayati. Workshop Pengembangan Sumber Daya Manusia Di Dalam Bidang Kimia Organik Bahan Alam Hayati. Padang: Universitas Andalas.
[6] Kadarisman I. 2000. Isolasi dan identifikasi senyawa kimia bioaktif dari Rimpang Bangle (Zingiber Cassumunar Roxb). Skripsi. Bogor: Institut Pertanian Bogor.
[7] Chozin A. 1996. Uji brine shrimp dan analisis kandungan kimia fraksi ekstrak methanol 95% daun Suren, Tuna Sureni (BL.). Prosiding Symposium Penelitian Bahan Obat Alami VIII. Perhitungan Penelitian Bahan Kimia Alami. Balitto: Bogor.
[8] Harborne J. B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: ITB.
[9] Meyer B. N. N. R. Ferrigni, J. E. Putman, L. B. Jacobsen, D. E. Nichol dan J. L Melaughlin. 1982. Brine Shrimp: A convenient general bioassay for active plant constituent. Planta Medika. 45: 31-34.
[10] Pebriana R. B, Bantari W. K.W, Widayanti E., Atifah N. S.W, Wijayanti T. R., Riyanto S. dan Meiyanto E. 2008. Pengaruh ekstrak metanol daun kenikir (Cosmos caudatus Kunth.) terhadap pemacuan apoptosis sel kanker payudara. PHarmacon. 9(1): 21-26.