UJI FITOKIMIA DAN TOKSISITAS (Brine Shrimp Lethality Test) EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN FRAKSI ETANOL SISA DARI DAUN AJERAN (Bidens pilosa L.)

  • Nuraini Meti Audia Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Daniel - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Eva Marliana Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University

Abstract

Phytochemical test and toxicity in ethanol extract, ethyl acetate fraction and residual ethanol fraction of ajeran leaves (Bidens pilosa L.) has been done. The method used to determine secondary metabolites is clualitative test and the value of Lethal Concentration (LC50) with the Brine Shrimp Lethality Test (BSLT). Phytochemical test results showed that the ethanol extract and ethyl acetate fraction contained flavonoid, steroid and phenol compound. Residual ethanol fraction contained flavonoid and phenol compound. Toxicity test showed ethanol extract had LC50 value of 862.19 ppm. Ethyl acetate fraction has LC50 value of 8.26 ppm. Residual ethanol fraction had LC50 value of 275.24 ppm. The results of the toxicity test can be concluded that the ethyl acetate fraction very toxic category, ethanol extract and residual ethanol fraction included toxic category. The results that obtained from GC-MS characterization were steroid

Keyword
Ajeran (Bidens pilosa L.), Phytochemical Test, Toxicity, Brine Shrimp Lethality Test, GC-MS.

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References

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Published
2021-01-06
How to Cite
AUDIA, Nuraini Meti; -, Daniel; MARLIANA, Eva. UJI FITOKIMIA DAN TOKSISITAS (Brine Shrimp Lethality Test) EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN FRAKSI ETANOL SISA DARI DAUN AJERAN (Bidens pilosa L.). JURNAL ATOMIK, [S.l.], v. 5, n. 2, p. 67-72, jan. 2021. ISSN 2549-0052. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/971>. Date accessed: 16 jan. 2021.
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Artikel