UJI BIOAKTIVITAS PADA EKSTRAK KASAR ETANOL, FRAKSI n-HEKSAN, ETIL ASETAT DAN ETANOL-AIR DARI DAUN ANDONG (Cordyline terminalis Kunth)

  • Ritson Purba Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
  • Enos Tangke Arung Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
  • Teddy Tranoto Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Abstract

Phytochemical, brine shrimp lethality and antibacterial activity tests in each fraction from Andong leaves extract(Cordyline terminalis Kunth) that came fromUnmul Samarinda botanical garden, Kalimantan Timur has been carried out. The leaf was extracted with ethanol andconcentrated by using rotary
evaporator. The crudeethanol extract was fractioned with n-hexane, and ethyl acetate. Based on the secondary metabolites phytochemical test of the Cordyline terminalis Kunth leaves show that crudeethanol extract is contain phenol, saponin and steroid.n-hexane fraction iscontain steroid.Ethylacetate fraction is contain phenol and steroid. And ethanol-water fraction is contain phenol and saponin. Brine shrimp lethality test exhibit mortality rate of Artemiasalina (L) by Probit SAS analysis to determine lethal concentration 50% (LC 50 ) value. The test showed that the most active wascrudeethanol extract with LC 50 value of 26.8788 ppm. Antibacteria activity test of extracts for Bacillus cereus bacteria (positiveGram) andEscherichia coli (negativeGram) were carried out by paper disk method. The test showed that the most active were ethyl acetate extract with minimum inhibitor consentration of 2-10% which clear zone diameter was 6,4 mm on Bacillus cereus bacteria and 6 mm on Escherichia coli
bacteria.
Keywords: Phytochemical, Antibacteria activity, LC 50

References

Ardiansyah. 2007. 28 Januari 2009. Antioksidan dan Peranannya Bagi Kesehatan. Artikel Iptek. http://ardiansyah.multiply.com/journal/item/14

Dalimarta, Setiawan. 2006. Atlas Tumbuhan Obat Indonesia Jilid 4. Jakarta: Puspa Swara

Darwis, D. 2000. Teknik Dasar Laboratorium dalam Penelitian Senyawa Bahan Alam Hayati. Workshop Pengembangan Sumber Daya Manusia di dalam Bidang Kimia Organik Bahan Alam Hayati. FMIPA UNAND,Padang.

Day, R. A. dan Underwood. A. L. 1998. Analisis Kimia Kuantitatif. Edisi Keenam. Jakarta : Erlangga.

Fessenden dan Fessenden. 1986. Kimia Organik. Jilid 2. Jakarta : Erlangga.

Hanani, Endang, Abdul Mun’im dan Ryany Sekarini. 2005. Identifikasi Senyawa Antioksidan dalam Spons Callyspongia SP dari kepulauan Seribu. Jurnal Majalah Ilmu Kefarmasian.

Harborne, J.B. 1987. Metode Fitokimia. Bandung : Penerbit ITB.

Hariaman, A. 2008. Tumbuhan Obat dan Khasiatnya. Jakarta : Penebar Swadaya.

Harun, N dan Syari W. 2002. Aktivitas antioksidan ekstrak daun dewa dalam menghambat sifat hepatotoksi khalotan dengan dosis sub anastesi pada mencit. Jurnal Sains dan Teknologi Farmasi. Padang : Genta Kirana Grafika, 7(2):63-70.

Herbert, R.B. 1995. Biosintesis Metabolisme Sekunder edisi kedua Penerjemah Bambang Srigendono. Semarang :IKIP Semarang Press.
Published
2014-05-18
How to Cite
PURBA, Ritson; ARUNG, Enos Tangke; TRANOTO, Teddy. UJI BIOAKTIVITAS PADA EKSTRAK KASAR ETANOL, FRAKSI n-HEKSAN, ETIL ASETAT DAN ETANOL-AIR DARI DAUN ANDONG (Cordyline terminalis Kunth). JURNAL KIMIA MULAWARMAN, [S.l.], v. 11, n. 2, may 2014. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/27>. Date accessed: 14 dec. 2017.
Section
Artikel