UJI BIOAKTIVITAS PADA EKSTRAK KASAR ETANOL, FRAKSI n-HEKSAN, ETIL ASETAT DAN ETANOL-AIR DARI DAUN ANDONG (Cordyline terminalis Kunth)
Abstract
Phytochemical, brine shrimp lethality and antibacterial activity tests in each fraction from Andong leaves extract(Cordyline terminalis Kunth) that came fromUnmul Samarinda botanical garden, Kalimantan Timur has been carried out. The leaf was extracted with ethanol andconcentrated by using rotaryevaporator. The crudeethanol extract was fractioned with n-hexane, and ethyl acetate. Based on the secondary metabolites phytochemical test of the Cordyline terminalis Kunth leaves show that crudeethanol extract is contain phenol, saponin and steroid.n-hexane fraction iscontain steroid.Ethylacetate fraction is contain phenol and steroid. And ethanol-water fraction is contain phenol and saponin. Brine shrimp lethality test exhibit mortality rate of Artemiasalina (L) by Probit SAS analysis to determine lethal concentration 50% (LC 50 ) value. The test showed that the most active wascrudeethanol extract with LC 50 value of 26.8788 ppm. Antibacteria activity test of extracts for Bacillus cereus bacteria (positiveGram) andEscherichia coli (negativeGram) were carried out by paper disk method. The test showed that the most active were ethyl acetate extract with minimum inhibitor consentration of 2-10% which clear zone diameter was 6,4 mm on Bacillus cereus bacteria and 6 mm on Escherichia coli
bacteria.
Keywords: Phytochemical, Antibacteria activity, LC 50
Downloads
References
Dalimarta, Setiawan. 2006. Atlas Tumbuhan Obat Indonesia Jilid 4. Jakarta: Puspa Swara
Darwis, D. 2000. Teknik Dasar Laboratorium dalam Penelitian Senyawa Bahan Alam Hayati. Workshop Pengembangan Sumber Daya Manusia di dalam Bidang Kimia Organik Bahan Alam Hayati. FMIPA UNAND,Padang.
Day, R. A. dan Underwood. A. L. 1998. Analisis Kimia Kuantitatif. Edisi Keenam. Jakarta : Erlangga.
Fessenden dan Fessenden. 1986. Kimia Organik. Jilid 2. Jakarta : Erlangga.
Hanani, Endang, Abdul Mun’im dan Ryany Sekarini. 2005. Identifikasi Senyawa Antioksidan dalam Spons Callyspongia SP dari kepulauan Seribu. Jurnal Majalah Ilmu Kefarmasian.
Harborne, J.B. 1987. Metode Fitokimia. Bandung : Penerbit ITB.
Hariaman, A. 2008. Tumbuhan Obat dan Khasiatnya. Jakarta : Penebar Swadaya.
Harun, N dan Syari W. 2002. Aktivitas antioksidan ekstrak daun dewa dalam menghambat sifat hepatotoksi khalotan dengan dosis sub anastesi pada mencit. Jurnal Sains dan Teknologi Farmasi. Padang : Genta Kirana Grafika, 7(2):63-70.
Herbert, R.B. 1995. Biosintesis Metabolisme Sekunder edisi kedua Penerjemah Bambang Srigendono. Semarang :IKIP Semarang Press.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
This license requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, even for commercial purposes. If others remix, adapt, or build upon the material, they must license the modified material under identical terms.