UJI TOKSISITAS AKUT EKSTRAK ETANOL BUAH LAKUM (Cayratia tryfolia) TERHADAP LARVA Artemia salina Leach DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

  • Retno Prasetya
  • Iis Intan W

Abstract

Lakum fruit use as spices could be hint for one of traditional herbal plant which using Brine Shrimp Lethality Test (BSLT) method in toxicity test. From the method, it will be know acute toxicity potential on lakum fruit’s extract against Artemia salina Leach larvae. This research was an experimental research using Post Test Only Control Group Design. It is used 160 larvaes as test animal which is divided on 4 groups, each group is done by the replication of research for 3 times and one control for each group. The various of concentration is 200 ppm, 400 ppm, 800 ppm, and 1600 ppm. The result is against larvae that died 24 hours after component test was given. Through the data, LC 50 value of ethanol extract of lakum fruit was analyzed by linear regression. Bassed on the researched, the value of LC 50 of ethanol extract of lakum fruit is172.38 μg/ml. The value of LC 50 is less than 1000 ppm. It’s mean that the extract had acute toxicity potential against Artemia salina Leach larvae.

Downloads

Download data is not yet available.

References

Andarwulan, dkk. 2011. Analisis Pangan. Jakarta: Dian Rakyat.

Cahyadi Roby. 2009. Uji Toksisitas Akut Ekstrak Etanol Buah Pare (Momordica charantia L.) Terhadap Larva Artemia salina Leach Dengan Metode Brine Shrimp Lethality Test (BST). Semarang: Fakultas Kedokteran Universitas Diponegoro.

Kumar D, dkk. 2011. A Review on Chemical and Biological Properties of Cayratia trifolia Linn. (Vitaceae). Phcog Rev 2011;5:184-8. Tersedia dihttp://www.phcogrev.com/article.asp?, diakses 10 November 2012.

Lenny Sovia, dkk. 2005. Isolasi dan Uji Bioaktivitas Kandungan Kimia Utama Puding Merah (Graptophyllum Pictum L. Griff) dengan Metode Uji Brine Shrimp. Sumatra Utara: Fakultas MIPA Universitas Sumatera Utara.

Masroh LF. 2010. Isolasi Senyawa Aktif dan Uji Toksisitas Ekstrak Heksana Daun Pecut Kuda (Stachytharpheta jamaicensis L.Vahl). Malang: Universitas Islam Negeri (UIN) Maulana Malik Ibrahim.

Nita Rochani, 2009. Uji Aktivitas Antijamur Ekstrak Daun Binahong (Anredera cordifolia (Tenore) Steen) Terhadap Candida albicans Serta Skrining Fitokimianya. Tersedia di http://www.geocities.com, diakses tanggal 20 februari 2012.

Sari Reski, 2012. Skrining Uji Toksisitas Dengan Metode Brine Shrimp Lethality Test (BSLT) Pada Larva Udang (Artemia salina Leach). Tersedia di http://rv-reskisari. blogspot.com/2012/06/uji-toksisitas-dengan-metode-brine.html, diakses tanggal 27 November 2012.

Widianti A, Suhardjono. 2010. Uji Toksisitas Akut Ekstrak Etanol Buah Cabai Rawit (Capsicumfrutescens) Terhadap Larva Artemia salina Leach Dengan Metode Brine Shrimp Lethality Test (BST). Semarang: Fakultas Kedokteran Universitas Diponegoro.
Published
2013-04-26
How to Cite
PRASETYA, Retno; W, Iis Intan. UJI TOKSISITAS AKUT EKSTRAK ETANOL BUAH LAKUM (Cayratia tryfolia) TERHADAP LARVA Artemia salina Leach DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT). PROSIDING SEMINAR NASIONAL KIMIA, [S.l.], apr. 2013. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/prosiding/article/view/114>. Date accessed: 29 mar. 2024.
Section
ARTIKEL

Keywords

Cayratia tryfolia; lakum; Brine Shrimp Lethality Test (BSLT); acute toxicity