UJI TOKSISITAS (brine shrimp lethality test) DAN PENENTUAN AKTIVITAS ANTIOKSIDAN ALAMI DARI DAUN TERAP (ArtocarpusodoratissimusB.) DENGAN METODE DPPH (2,2-diphenyl-1-picrylhidrazyl)
Abstract
The phytochemical test, brine shrimp lethality test and antioxidant activity test on secondary metabolits of terap (Artocarpus odoratissimus B.)leaves have been completed. The leaves samples are extracted by masseration method that is concentrated by using rotary evaporator. The total extract are fractioned with n-hexane and ethyl acetate. Based on secondary metabolits phytochemical test of terap (Artocarpus odoratissimus B.)leaves showed that total extract, n-hexane fraction extract andEthyl Acetat fraction extractcontains steroid and flavonoid. In brine shrimp lethality test, the increase larvae death data was recorded and processed using SAS Probit Analysis to determine the Lethal Concentration 50 % (LC 50 ) value. The results of this test showed that the most active extract is metanol extract with LC 50 value of 110,5176ppm. Based on the antioxidant activity by scavenging activity of DPPH used spectrophotometry was obtained that Inhibition Concentration 50% (IC 50 ) of total extract is 563,57 ppm, extract of n-hexane fraction is 631,87 ppm and extract of ethyl acetat fraction is 45,30 ppm.Downloads
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References
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Fadzelly A.B., Mohd,Maryati Mohamed, Datin,Asmah Rahmat,Burr, Steven A., Fry and Jeffrey R. 2009. Phytochemical and Antioxidant Activity of Different Parts of Bambangan (Mangifera Pajang) and Tarap (Artocarpus odoratissimus).Food Chemistry, 133(2). Pp. 479-483. ISSN 0308-8146.
Harborne, J.B. 1987. Metode Fitokimia. Bandung: ITB.
Kalt, W., Forney, C.F., Martin, A., and Prior, R.L. 1999. Antioxidant capacity, vitamin C, phenolics and anthocyanins after fresh storage of small fruits. Journal of Agricultural and Food Chemsitry, 47: 4638-4644.
Khan, M.R., Omoloso Ad and Kihara M. 2003.Antibacterial activity of Artocarpus heterophyllus. Fitoterapia 74: 501-505.
Kikuzaki, K and Nakatani, N. 1993. Antioxidant Effects of Some Ginger Constituents, J. F Food. Sci. 58:1407-1410
Meyer, B.N., Ferigni, N.R., Putnam, J.E., Ja Cobsen, L.B., Nichols, D.E and McLaughlin, J. L. 1982. Brine Shrimp, A Convenient General Bioassay for Active Constituents. Planta Medica45: 31-34.
Nasution, R. 2013. Isolasi dan Penentuan Struktur Senyawa Steroid dari Daun Tumbuhan Kulu (Artocarpus camansi: Sukun Berbiji) yang Bersifat Antidiabetes. Disertasi. Medan: Universitas Sumatra Utara.
Nomura, T and Hano, Y. 1998. Isoprenoid Substituted Flavanoid from Artocarpus Plants (Moracea) Heterocycles, 47,1179.
Ramadhan, R. 2012. Potensi Tumbuhan Annatto (Bixa Orellana L.)Sebagai Tumbuhan Obat dan Pewarna Alami. Tesis Fakultas Kehutanan, Universitas Mulawarman Samarinda.
Robinson, T. 1995. Kandungan Organik Tumbuhan Tingkat Tinggi. Bandung:ITB.
Verheij, E.W., M and Coronel, R.E (eds). 1992. Plant Resources of South Asia No. 2 Edible Fruits and Nuts. Bogor : Prosea Foundation.
Widyawaruyanti, A., Subehan, Kalauni S.K., Awale, S., Nindatu, M., Zaini, N.C., Syafrudin, D., Asih, P.B.S., Tezzuka, Y and Kadota, S. 2007. New Prenylated Flavones from Artocarpus champenden,, and Their Antimalarial Activity In Vitro. J Nat Med 61; 410-413
Published
2016-04-26
How to Cite
SEPTIANI, Tri Wahyu; -, Erwin.
UJI TOKSISITAS (brine shrimp lethality test) DAN PENENTUAN AKTIVITAS ANTIOKSIDAN ALAMI DARI DAUN TERAP (ArtocarpusodoratissimusB.) DENGAN METODE DPPH (2,2-diphenyl-1-picrylhidrazyl).
PROSIDING SEMINAR NASIONAL KIMIA, [S.l.], apr. 2016.
Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/prosiding/article/view/124>. Date accessed: 24 apr. 2024.
Section
ARTIKEL
Keywords
Leaves of terap(Artocarpus odoratissimus B.), Phytochemical test, antioxidant activity test and DPPH
