DETEKSI DOPAMIN SECARA VOLTAMETRI MENGGUNAKAN ELEKTRODA PASTA KARBON TERMODIFIKASI ETER MAHKOTA (DIBENZO-18-CROWN-6)
Abstract
The research about the detection of dopamine by voltammetry method using crown ether modified carbon paste electrode has been carried out. The aims of this research are to investigate the influence of pH and the composition of modifier to the sensitivity and limit of detection for dopamine analysis. The measurement was conducted by differential pulse voltammetry method.The optimum of pH and modifier (crown ether) composition in the detection of dopamine are 4,0 and 0.8%, respectively. The linear concentration range is obtained from 10 to 200 µM, linear regression equation y = 4,968 + 0,068x with R2 = 0,9988 and limit of detection 6,865 x 10-6 M. The modified carbon paste electrode has relative standard deviation smaller than Horwitz’s (1,28%<5,66%), therefore this electrode has a good reproducibility. The recovery using crown ether-modified carbon paste electrode is 99,0% showing a good accuration. The measurement of commercial samples contain dopamine HCl (Giulini and Proinfark injection) is 87,0% and 91,5% compared with the composition on the label
Downloads
References
Bradshaw, J.S., and Izzat, R.M., 1997, Crown Ethers : The Search for Selective Ion Ligating Agents. Acc. Chem. Res., 30:338-345
Downard, A.J., Roddick A.D., and Bond, A.M., 1995, Covalent Modification of Carbon Electrodes for Voltammetric Differentiation of Dopamine and Ascorbic Acid, Anal. Chim. Acta, 317:303–310
Dursun, Z., Pelit, L., and Taniguchi, I., 2009, Voltammetric Determination of Ascorbic Acid and Dopamine Simultaneously at a Single Crystal Au(III) Electrode, Turky J. Chem 33:223 – 231
Hows, M.E.P., Lacroix, L.C., Heidbreder, A.J., Organ, A.J., and Shah, 2004, Highperformance Liquid Chromatography/Tandem Mass Spectrometric Assay for the Simultaneous Measurement of Dopamine, Norepinephrine, 5-Hydroxytryptamine and Cocaine in Biological Samples, J. of Neurosc. Method,138:123– 132
Kang, T.F., Shen, G.L., and Yu, R.Q., 1997, Voltammetric Behaviour of Dopamine at Nickel Phthalocyanine Polymer Modified Electrodes and Analytical Applications,Anal. Chim. Acta, 356:245– 251
Kutner, W., Wang, J., L’her, M., and Buck, R.P., 1998, “Analytial Aspect of Chemically Modified Electrodes: Classification, Critical Evaluation and Recommendations”, Pure and Appl. Chem., 70:1301-1318
Lamb, J.D., Lazatt, R.M., Cristensen, J.J., Eatough, D.J., 1979, Coordination Chemistry of Macrocyclic Compounds, Plenum, New York
Quan, D.P., Tuyen, D.P., Lam, T.D., Tram, P.T.N., Binh, N.H., and Viet, P.H., 2011, Electrochemically Selective Determination of Dopamine in the Presence of Ascorbic Acid and Uric Acid on the Surface of the Modified Nafion/Single Wall Carbon Nanotubes/Poly(3-methylthiophene) Glassy Carbon Electrodes, Colloids and Surfaces B: Biointerfaces, 88:764-770
Raoof, J.B., Ojani, R., and Nadimi, S.R., 2004, Preparation of Polypyrolle/Ferrocyanide Film Modified Carbon Paste Electrode and Its Application on the Electrocatalytic Determination of Ascorbic Acid, Electrochim. Acta, 49:271-280
Rivera, C., and Rodriguez, R., 2011, Horwitz Equation As Quality Benchmark, Testing Laboratory, Bufete de Ingenieros Industriales S.C., Chihuahua, ISO/IEC 17025
Sabbioni, C., Saracino M.A., Mandrioli, R., Pinzauti, S., Furlanetto, S., Gerra, G., and Raggi, M.A., 2004, Simultaneous Liquid Chromatographic Analysis of Catecholamines and 4-Hydroxy-3-Methoxyphenylethylene Glycol in Human Plasma : Comparison of Amperometric and Coulometric Detection, J. of Chromatography A 1032:65–71
Suw, Y. L., 2006, Detection of Dopamine in The Pharmacy With a Carbon Nanotube Paste Electrode Using Voltammetry, Bioelectrochemistry, 68:227 – 231
Ulubay, S., and Dursun, Z., 2010, Cu Nanoparticles Incorporated Polypyrrole Modified GCE for Sensitive Simultaneous Determination of Dopamine and Uric Acid, Talanta, 80:1461-1466
Wang, Q., Jiang, N., Li, N., 2001, Electrocatalytic Response of Dopamine at a Thiolactic Acid Self-Assembled Gold Electrode, Microchemical Journal, 68:77– 85
Wang, J., 2000. Analytical Electrochemisty, 2nd Ed.,. Wiley-VCH. New York ISBN: 0-471-22823-0 (Electronic)
Workman, J., and Howard, M., 2006, Limitations in Analytical Accuracy, part I: Horwizt’s Trumpet,21(9) : 18-24
Zhang, H.N., Liu, N.Q., and Zhu, Z., 2000, Electrocatalytic Response of Dopamine at DL-Homocysteine Self-Assembled Gold Electrode,Microchemical Journal, 64:277– 282
Zhao, H., Zhang, Y., and Yuan, Z., 2001, Study on the Electrochemical Behavior of Dopamine with Poly(sulfosalicylic acid) Modified Glassy Carbon Electrode,Anal. Chim. Acta, 441:117–122
Zlatuse, D.P., David, C.C., Christopher, R.B., Milton, L.L., and Steven, W.G., 2002, Determination of Catecholamines and Metanephrines in Urine by Capillary Electrophoresis-Electrospray Ionization, J. of Chrom. B: Biomedical Sciences and Applications, 776:221– 229Peraturan Pemerintah, (1999)
Keywords
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
This license requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, even for commercial purposes. If others remix, adapt, or build upon the material, they must license the modified material under identical terms.
BY: Credit must be given to you, the creator.
SA: Adaptations must be shared under the same terms.
