ISOLASI SENYAWA FENOLIK PADA FRAKSI METANOL-AIR DARI UMBI TUMBUHAN SARANG SEMUT (Myrmecodia tuberose Jack)
Abstract
The Isolation of phenolic compound in methanol-water fraction from Myrmecodia tuberosa Jack that come from Bulungan have been done. The air-dried powdered from corm of ant plant (Myrmecodia tuberosa Jack) (250 gram) were extracted by methanol at room temperature (27 oC) and fractioned with n-hexane then ethyl acetate. Partition of phenolic compounds in Methanol-Water fraction was chromatographed on silica gel column (35–70 mesh) that eluted with Isocratic method and gave 10 fractions as a result, that fractions were A, B, C, D, E, F, G, H, I dan J fraction. C; E; G and H (alliance fraction) gave the best spot partitioned and the spot was not fluorescence. Purification of alliance fraction gave yellow-brown crystal with retention factor 0.52 that eluted with methanol : ethyl acetat (8:2) whereas the retention factor in methanol 0.82 and ethyl acetate 0,33. Infrared spectrum of alliance fraction shown absorption at value : 3351.83; 2945.33; 2833.04; 1741.00; 1451.60; 1215.67; 885.74 and 776.74 cm-1 with melting point 224 - 228oC. UV and Visible spectrum of alliance fraction shown wavelength at value 263 and 334 nm. Phenolic compounds in Methanol-Water fraction from the corm of ant plant (Myrmecodia tuberosa Jack) was predictioned as phenolic compound of flavonoid group.
Keywords : Ant Plant (Myrmecodia tuberosa Jack), Phenolic, Isocratic , Flavonoid, Isolation,
Downloads
References
Efdi, M. 1999. Isolasi Alkaloid dari Daun Tumbuhan Ophiorrhiza cf. kunstleri King. Makalah Seminar Hasil Program Pasca Sarjana. Padang. Universitas Andalas.
Harborne, I.B., 1987. Metode Fitokimia (Penuntun Cara Modern Menganalisis Tumbuhan). Terjemahan K. Padmawinata dan I. Soediso. Bandung : ITB.
Mulja, H. M. dan Suharman. 1995. Analisis Instrumental. Surabaya : Universitas Airlangga – Press.
Robinson, T. 1995. Kandungan Kimia Organik Tumbuhan Tinggi. Bandung: ITB.
Subroto, M. A. 2006. Gempur Penyakit dengan Sarang Semut. Jakarta : Penebar Swadaya.
Subroto, M. A. 2006. Sebuah Pedang Bermata Dua. http://www.trubus-online. com/argtj?id=p.
Sudjadi, M. S. 1983. Penentuan Struktur Senyawa Organik. Bandung: Ghali Indonesia.
Suriawiria, U. 2000. Obat Mujarab dari Pekarangan Rumah. Jakarta : Papas Sinar Sinanti.
Vogel. 1990. Buku Teks Analisis Anorganik Kualitatif Makro dan Semimikro. Jilid I. Terjemahan L. Setyono dan A. Hadyana Pudjaatmaka. Jakarta : Kalman Media Pusaka.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
This license requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, even for commercial purposes. If others remix, adapt, or build upon the material, they must license the modified material under identical terms.