ISOLASI AMILASE DARI KECAMBAH BIJI NANGKA (Artocarpus heterophyllus Lam)

  • Ismi Fahmi Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
  • Winni Astuti Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
  • Saibun Sitorus Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Abstract

This research aims to isolate the crude extract amylase of sprouted seeds jackfruit (Artocarpus heterophyllus Lam) and determine the specific activity. Determination of total protein concentration and amylase activity of enzyme crude extract on seed germination jackfruit (Artocarpus heterophyllus Lam). Determination of total protein concentration using the method of Bradford, whereas the determination of amylase activity was performed using dinitrosalisilat acid method (DNS). The results showed a total protein concentration of the enzyme crude extract of sprout seeds jackfruit (Artocarpus heterophyllus Lam) is 319,47 ug / mL, amylase activity of 13,96 U / mL and specific activity is 1748,05 U / mg.

 

Published
2017-03-01
How to Cite
FAHMI, Ismi; ASTUTI, Winni; SITORUS, Saibun. ISOLASI AMILASE DARI KECAMBAH BIJI NANGKA (Artocarpus heterophyllus Lam). JURNAL ATOMIK, [S.l.], v. 2, n. 1, p. 140-142, mar. 2017. ISSN 2549-0052. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/362>. Date accessed: 24 sep. 2017.
Section
Artikel