INHIBISI XANTIN OKSIDASE OLEH FRAKSI ETIL ASETAT DARI DAUN JARUM TUJUH BILAH (Pereskia bleo (Kunth) D.C) SEBAGAI ANTIHIPERURISEMIA

Prinka Susmita Sari; Saibun Sitorus; Rahmat Gunawan

Prinka Susmita Sari Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Saibun Sitorus Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Rahmat Gunawan Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman

Abstract

“Jarum Tujuh Bilah” leaves (Pereskia bleo (Kunth) DC) are a medicinal plant that often used in traditional medicine to reduce uric acid levels. This plant contains secondary metabolites which have the potential to inhibit xanthine oxidase activity. In this study phytochemical tests were conducted on ethanol extract and ethyl acetate fraction of “Jarum Tujuh Bilah” leaves (Pereskia bleo (Kunth) DC), then isolated xanthine oxidase from fresh cow's milk, and the activity of ethyl acetate fraction against xanthine oxidase. The results showed that ethanol extract contained flavonoid, alkaloid and phenolic compounds. Then ethyl acetate fraction contained flavonoid and alkaloid compounds. The value of xanthine oxidase activity from the isolation of xanthine oxidase is 0.00063 U/mL in the fractionated enzyme and 0.00025 U/mL in the supernatant. The inhibition of 92.06% ethanol extract and the inhibition of ethyl acetate fraction was 88.89%.

Keyword

“Jarum Tujuh Bilah” leaves, xanthine oxidase, allopurinol

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References

[1] Harbone, J.B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: ITB.
[2] Robinson, T. 1995. Kandungan Organik Tumbuhan Tinggi. (Terjemahan). Bandung: Penerbit ITB.
[3] Surahman, Agus. 2012. Uji Fitokimia dan Daya Inhibisi Ekstrak Daun Sendok (Plantago major L,.) dan Buah Srikaya (Annona squamosa L,.) terhadap Aktivitas Xantin Oksidase. Jurnal. Malang: Universitas Negeri Malang.
[4] Susanti, A. 2011. Pengaruh Ekstrak Tempuyung (Sonchus arvensis) Terhadap Aktivitas Xantin Oksidase Secara In Vitro Sebagai Dasar Uji Kinetika. Jurnal. Bogor: Departemen Kimia Institut Pertanian Bogor.
[5] Wulandari, Sri. 2012. Inhibisi Xantin Oksidase oleh Ekstrak Etanol Kulit Melinjo (Gnetum gnemon) Relatif Terhadap Allopurinol. Jurnal. Malang: Universitas Negeri Malang.