ISOLASI BAKTERI ENDOFIT PENGHASIL LIPASE PADA DAUN GINSENG JAWA (Talinum triangulare (Jacq.) Willd)

  • Istianti Irfa'a Nur Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman, Jalan Barong Tongkat, Kampus Gn. Kelua, Samarinda 75123, Kalimantan Timur, Indonesia
  • Winni Astuti Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
  • Rudi Kartika Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman

Abstract

Endophytic bacteria are bacteria that live in plant tissues. These bacteria produce extracellular enzymes, one of which is the lipase. This research was conducted to isolate the endophytic bacteria of Ginseng Jawa (Talinum triangulare (Jacq.) Willd) leaves which produced lipase and lipase screening from these bacteria. Isolation was carried out on nutrient agar (NA) media and screening of endophytic bacteria were carried out on nutrient agar (NA) media containing olive oil and Rhodamin B. Isolation results obtained by 5 single colonies and one colony of positive endophytic bacteria that produces lipase which characterized by the presence of orange-colored luminescence on the surface of the bacterial colony

Keyword
Endophytic Bacteria, Talinum triangulare (Jacq.) Willd, Screening, Lipase.

Downloads

Download data is not yet available.

References

[1] Munif A., dan Harni R. 2011. Keefektifan bakteri endofit untuk mengendalikan nematoda parasit Meloidogyne incognita pada tanaman lada. Buletin Riainy STRI, 2(3):377–82.
[2] Hung P. Q., dan Annapurna K. 2004. Isolation and characterization of endophytic bacteria in soybean (Glycine sp.). Omonrice, 12:92-101.
[3] Yasinok A. E., Sahin F. I., dan Heberal M. 2008. Isolation of endophytic and xylanolitic Bacillus pumilus strain from Zea Mays. Tarim Bilimleri Dergisi, 14(4):384-380.
[4] Palmer, Trevor. 1985. Understanding enzyme. Ellishorwood Publisher.
[5] Sumarlin L. O., Mulyadi D., dan Asmara Y. 2013. Identifikasi potensi enzim lipase dan selulase pada sampah kulit buah hasil fermentasi. Jurnal Ilmu Pertanian Indonesia (JIPI) 18(3):159–66.
[6] Susanty A., Fitriani, dan Candra K.P. 2013. Isolasi bakteri penghasil lipase dengan kemampuan esterase untuk produksi metil ester. J. Res. Tech. Ind., hal 134-142.
[7] Carrim A. J. L., Barbosa E.C., dan Vieira J. D. G. 2006. Enzymatic activity of endophytic bacterial isolated of Jacaranda decurrens Cham (Carobinha-d-campo). Jurnal Braz. Arch. Biol. Technol., 49(3):353-359.
[8] Djafar F., Purwandaria T., dan Sinurat A. P. 2010. Isolation of endophytic bacteria from palm oil fruits and characterization of their lipases. Microbiol., 4(2):1-6.
[9] Pricilia S., Astuti W., dan Marliana E. 2018. Skrining bakteri endofit penghasil amilase, lipase dan protease dari daun Macaranga Hullettii King Ex Hook . F. Jurnal Atomik, 3(2):102–5.
Published
2021-03-31
How to Cite
NUR, Istianti Irfa'a; ASTUTI, Winni; KARTIKA, Rudi. ISOLASI BAKTERI ENDOFIT PENGHASIL LIPASE PADA DAUN GINSENG JAWA (Talinum triangulare (Jacq.) Willd). JURNAL ATOMIK, [S.l.], v. 6, n. 1, p. 53-55, mar. 2021. ISSN 2549-0052. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/848>. Date accessed: 29 oct. 2021.
Section
Artikel