SPECTROPHOTOMETRIC ANALYSIS OF CHLORAMPENICOL IN TIGER PRAWNS (PENAEUS MONODON) BASED ON THE DIAZOTATION REACTION

ANALISIS CHLORAMPENICOL PADA UDANG WINDU (PENAEUS MONODON) SECARA SPEKTROFOTOMETRI BERBASIS REAKSI DIAZOTASI

  • Moh. Syaiful Arif Department of Chemistry, Mulawarman University
  • Indra Kurniawan Department of Chemistry, Mulawarman University
  • Bohari Yusuf Department of Chemistry, Mulawarman University
DOI: https://doi.org/10.30872/jkm.v20i2.1017

Abstract

Spectrophotometric analysis of chloramphenicol in tiger prawns (Penaeus Monodon) based on the diazotation reaction has been carried out. Sulfanilamide is used as a source of diazo salt to be coupled with chloramphenicol. In this study, several optimizations were carried out such as optimization of temperature, volume of HCl, volume of NaNO2, volume of sulfanilamide and contact time. Diazonium salt coupled with sulfanilamide produces a clear  yellow compound with a maximum wavelength of 400.941 nm. The results of the analysis chloramphenicol on tiger shrimp, obtained the coefficient of variation  were 4.026 %; 1.951 %; and 4.277 %.. Accuracy ranges from 99.7143% to 100.8730%. The Limit of Detection value is 6.24 mg/mL and the Limit of Quantization value was 20.803 mg/mL. The sensitivity value is 0.0021 mL /mg.

Keyword
Chlorampenicol, reaksi diazotasi, udang windu

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Published
2023-05-26
How to Cite
ARIF, Moh. Syaiful; KURNIAWAN, Indra; YUSUF, Bohari. SPECTROPHOTOMETRIC ANALYSIS OF CHLORAMPENICOL IN TIGER PRAWNS (PENAEUS MONODON) BASED ON THE DIAZOTATION REACTION. JURNAL KIMIA MULAWARMAN, [S.l.], v. 20, n. 2, p. 85-91, may 2023. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/1017>. Date accessed: 26 apr. 2024. doi: https://doi.org/10.30872/jkm.v20i2.1017.
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Vol 20 No 2 (2023)
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