VALIDASI METODE ANALISIS KADAR TIMBAL (Pb) DALAM MADU ALAM DI KALIMANTAN TIMUR
Abstract
Analysis method for determining Pb content in natural honey have been developed and validated. Research was done by analyzing one natural honey sample collected from Teritip (Balikpapan) with standard analysis method and three developed analysis method which their analytical performance tested later. Pb content was determined with atomic absorption spectrometry after dry digestion (method A) or wet digestion (method B (HNO3-H2O2; 3:8), C (HNO3-H2O2; 2:1) and D (HNO3)). Analytical performance parameter e. G. Linearity, working range, limit of detection, limit of quantitation, precision and accuracy were tested. Content of Pb in natural honey sample which analyzed with method A, B, C and D were found at 0.7923 ± 0.0311 µg/g, 0.7944 ± 0.0216 µg/g, 0.7998 ± 0.0218 µg/g and 0.7912 ± 0.0160 µg/g respectively. Analytical performance of four tested analytical method were still acceptable. Significance testing result showed that there were no significant differences in precision and accuracy between developed analysis method and standard analysis method, execpt for method D. Precision of method D was significantly better than standard analysis method.
Keywords: Honey, Lead, Method Validation
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References
BSN. 1998. SNI 01-2896-1998, Cara Uji Cemaran Logam dalam Makanan. Jakarta: Badan Standarisasi Nasional.
BSN. 2004. SNI 01-3545-2004, Madu. Jakarta: Badan Standarisasi Nasional.
Naidu, R. 2008. Chemical Bioavailability in Terrestrial Environments. Amsterdam: Elsevier.
Porrini, C., A. G. Sabatini, S. Girotti, S. Ghini, P. Medrzycki, F. Grillenzoni, L. Bortolotti, E. Gattavecchia dan G. Celli. 2003. Honey Bees and Bee Products as Monitors of the Environmental Contamination. Apiacta 38, 63-70.
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