SKRINING LIPASE DARI BAKTERI AIR BENDUNGAN BENANGA LEMPAKE KECAMATAN SAMARINDA UTARA DAN POTENSINYA SEBAGAI BAHAN ADITIF DETERGEN
Keywords:
Lipase, Bacteria, Water of the Benanga Lempake DamAbstract
Lipase is an enzyme that can hydrolyze triglycerol into glycerol and free fatty acids. The aim of this research is to obtain lipase-producing bacteria and determine the potential of lipase from water bacteria in the water of the Benanga Lempake Dam, North Samarinda for biodetergent applications through stability test of lipase activity against several commercial detergents. Screening of lipase-producing bacteria was carried out on nutrient agar media containing olive oil and Rhodamine B. The results showed that seven isolates were capable of producing lipase. Based on the stability test of lipase on detergent, it can be seen that the activity of lipase decreased by more than 70% at the addition of 1% detergent.
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References
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Sharma R, Chisti Y, Banerjee UC. 2001. Production, purification, characterization, and applications of lipases. Biotechnology Advances 19:627–662.
Lienfield, W. M., O’Brien, D. J., Serota, S., Barauskas, R. A. 1984. Lipid-Lipase Interaction I. Fat Splitting With Lipase from Candida Rogusa. Journal of the American Oil Chemists' Society, 61(6), 1067-1071.
Murni, S. W., Kholisoh, S. D., and Petrissia, E.M. 2011. Produksi, Karakterisasi, dan Isolasi Lipase dari Aspergillus niger. 104(2011): 62-274.
Kasipah C. Sinta R dan Zeily N. 2013. Isolasi dan Karakterisasi Bakteri Penghasil Enzim Lipase Ekstraseluler dari Lumpur Aktif Instalasi Pengolahan Air Limbah Industri Tekstil. Jurnal Ilmiah Arena Tekstil 28(1):1–46. Kelompok Keahlian Biokimia FMIPA, ITB, Balai Besar Tekstil, Bandung.
Chandra, P., Enespa, R.Singh dan P.K. Arora. 2020. Microbial Lipases and Their Industrial Applications: a Comprehensive Review. Microbial Cell Factories. 19(169).
Sharma R, Chisti Y, Banerjee UC. 2001. Production, purification, characterization, and applications of lipases. Biotechnology Advances 19:627–662.
Lienfield, W. M., O’Brien, D. J., Serota, S., Barauskas, R. A. 1984. Lipid-Lipase Interaction I. Fat Splitting With Lipase from Candida Rogusa. Journal of the American Oil Chemists' Society, 61(6), 1067-1071.
