ISOLASI DAN KARAKTERISASI SENYAWA FLAVONOID PADA FRAKSI ETIL ASETAT DAUN Macaranga lamellata Whitmore

  • Sherly Indah Puspita FMIPA UNMUL
  • Eva Marliana Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Daniel Daniel Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University

Abstract

Isolation and characterization of flavonoid compounds from ethyl acetate fraction of Macaranga lamellata Whitmore leaves have been done in this research. Macaranga lamellata Whitmore leaves was macerated using methanol and fractionated with n-hexane and ethyl acetate. The separation process of ethyl acetate fraction was conducted using gravity column chromatography by Step Gradient Polarity (SGP) method. The isolate was tested for its purity using thin layer chromatography with various eluents i.e. n-hexane, chloroform, methanol, acetone and ethyl acetate, with Rf values were 0; 0,7857; 0,8333; 0,9048 and 0,9286 respectively. The result indicated that the isolate was obtained as ML1 (3 mg) belong to flavonoid compound in the form of oil. Analysis of ML1 compound using UV displayed 2 peaks at ? 291 nm (band II) and 342 nm (band I). Analysis of ML1 compound using FT-IR displayed functional groups -OH, C-H aromatic, C-H aliphatic, C=C aromatic, C=O, C-OH and C-O-C. Based on the results of UV and FT-IR spectra, ML1 compound was suspected to contain flavonoid compound.

Keyword
Flavonoid, isolation, Macaranga lamellata Whitmore

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Published
2019-03-30
How to Cite
PUSPITA, Sherly Indah; MARLIANA, Eva; DANIEL, Daniel. ISOLASI DAN KARAKTERISASI SENYAWA FLAVONOID PADA FRAKSI ETIL ASETAT DAUN Macaranga lamellata Whitmore. JURNAL ATOMIK, [S.l.], v. 4, n. 1, p. 1-5, mar. 2019. ISSN 2549-0052. Available at: <https://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/630>. Date accessed: 13 july 2024.
Section
Artikel