Antifeedaant Compound From Daruju Leaves (Acanthus Ilicifolius Linn) Against Eggplant Leaves Beetle (Epilachna Sparsa)
Abstract
Daruju (Acanthus ilicifolius Linn) contain flavonoids, terpenoids, steroids, saponins, alkaloids and tannins that can be used as antifeedant compounds. The purpose of this study is to identify the active compound antifeedant from daruju leaves against the eggplant leaf beetle (Epilachna sparsa). The antifeedant activity test was carried out on the methanol extract, the partitioned fraction and the isolate. This study was began by maceration of daruju leaf powder (1000 g) with methanol that obtained methanol extract of 130.3 g. The methanol extracts were then separated by partitioning that obtained n-hexane, dichloromethane, ethyl acetate and methanol fraction. Antifeedant activity test against eggplant leaf beetle (Epilachna sparsa) showed the dichlormethane fraction at a concentration of 6 ppm had the best activity compared to other fractions with an activity of 90.62%. Separation and purification of the dichloromethane fraction to obtain isolation was carried out by column chromatography. The isolate showed 96.1% antifeedant activity at a concentration of 6 ppm. Based on 1H-NMR spectrum showed isolate is p-Oxy-2-ethylhexyl benzaldehyde.
Downloads
References
Fessenden, R., & Fessenden, J. (1986). Kimia Organik Dasar, Edisi 2. Jakarta: Erlangga.
Haji, A. G., Mas’ud, Z. A., & Pari, G. (2012). Identifikasi Senyawa Bioaktif Antifeedant Dari Asap Cair Hasil Pirolisis Sampah Organik Perkotaan. J. Bumi Lestari, 12 (1):1-8.
Handayani, S., Najib, A., & Wati, N. P. (2018). Uji Aktivitas Antioksidan Ekstrak Daun Daruju (Acanthus ilicifolius Linn.) dengan Metode Peredaman Radikal Bebas 1,1-Diphenyil-2-Picrylhidrazil (DPPH). Jurnal Fitofarmaka Indonesia, 5(2): 299:308.
Mandana, M., Puspawati, N., & Santi, S. (2013). Identifikasi Golongan. J. Kimia, 7 (1): 39-48.
Pradani, F. Y. (2009). Indeks Pertumbuhan Larva Aedes aegypti L. Yang Terdedah Dalam Ekstrak Air Kulit Jengkol (Pithecellobium lobatum). J. Aspirator, vol. 1(2): 81-85.
Saptiani, G., Prayitno, S. B., & Sutrisno, A. (2012). Aktivitas Antibakteri Ekstrak Jeruju (Acanthus ilicifolius) Terhadap Pertumbuhan Vibrio herveyi Secara in Vitro. J. Veteriner, 13(3): 257-262.
Shukla, P., Vidyasagar, P., Aldosari, S., & Abdel-Azim, M. (2012). Antifeedant Activity of Three Essential Oils Against the Red Palm Weevil, Rhynchophorus Ferrugineus. Bulletin of Insectology, 65 (1): 71-76.
Suhatri, M., Ardiningsih, P., & Widiyantoro, A. (2018). Senyawa Sitotoksik dari Fraksi Diklorometana Daun Daruju (Acanthus Ilicifolius Linn.) terhadap Sel HeLa. Jurnal Kimia Khatulistiwa, 7(2): 75-81.
Wardani, R. S., Mifbakhuddin, & Yokorinanti, K. (2010). Pengaruh Konsentrasi Ekstrak Daun Tembelekan (Lantana Camara) Terhadap Kematian Larva Aedes aegypti. J. kesehat Masy Indones, Vol 6(2): 30-38.
Yuantari, M. G. (2015). Analisis Resiko Pejananan Peptisida Terhadap Kesehatan Petani. Jurnal Kesehatan Masyarakat, 239-245.
Yuantari, M. G., Widianarko, B., & Sunoko, H. R. (2015). Analisis Resiko Pajanan

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.