UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI DARI TANAMAN MERKUBUNG (Macaranga gigantea (Rchb.f. & Zoll) Mull. Arg.) DENGAN EKSTRAK TOTAL, FRAKSI n-HEKSANA, ETIL ASETAT DAN ETANOL AIR TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus
Abstract
Phytochemical tests, mortality of larvae shrimp (brine shrimp lethality test) and the antibacterial activity of theleaf merkubung (Macaranga gigantea Rchb.f. & Zoll.) With various factions has been conducted. Merkubung leaf
samples were extracted with ethanol and concentrated by using rotary evaporator. The total extract was fractionated
with n-hexane and ethyl acetate. The results of phytochemical compounds secondary metabolites merkubung leaves
showed that the total extract are contains alkaloids, phenolics and steroids. n-hexane fraction are containing alkaloids,
flavonoids and steroids. Ethyl acetate faction are containing flavonoids and steroids. Ethanol-water faction is containing
phenolic compounds. Shrimp larvae mortality trials showed mortality rates of larvae of Artemia salina (L) by using
SAS Probit analysis to determine the value of Lethal Concentration 50 % (LC50). The test shows that the most toxic
fraction is the total extract with LC50 values of 459.8414 ppm. The test of antibacterial activity using paper disc method
and shows that the most active faction is the total extract with a MIC (Minimmum Inhibitor Concentration) of 1 % is
6.5 mm diameter clear zone on Staphylococcus aureus (Gram positive) and 6.25 mm in the bacterium Escherichia coli
(Gram negativ).
Keywords : Macaranga gigantea Rchb.f. & Zoll., phytochemical test, Antibacteria activity test, LC50.
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Published
2014-11-24
How to Cite
APRIANI, Azhar Seli; SALEH, Chairul; -, Alimuddin.
UJI FITOKIMIA DAN AKTIVITAS ANTIBAKTERI DARI TANAMAN MERKUBUNG (Macaranga gigantea (Rchb.f. & Zoll) Mull. Arg.) DENGAN EKSTRAK TOTAL, FRAKSI n-HEKSANA, ETIL ASETAT DAN ETANOL AIR TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus.
JURNAL KIMIA MULAWARMAN, [S.l.], v. 12, n. 1, nov. 2014.
ISSN 2476-9258.
Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/17>. Date accessed: 19 apr. 2024.
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