ISOLASI, IDENTIFIKASI DAN UJI TOKSISITAS SENYAWA FLAVONOID FRAKSI KLOROFORM DARI DAUN TERAP (ARTOCARPUS ODORATISSIMUS BLANCO)
Abstract
The research about the characterization of flavonoid compound from the leaves of plants applicability chloroform(artocarpus odoratissimus Blanco). Extraction plants done by maceration method and separation of compounds was
performed using the technique of vakum liquid chromatography and chromatography colomn flash. Separation result
obtained yellowish-white powder. Resul spectrum UV-visible absorption obtained namely the 254.37 to 181.28 area
absorbance wavelength (nm), while FT-IR analysis result provide absorption at wavenumber there is an alkyl group, a
hydroxyl group, an aromatic group and C-O-C stretching based on the data spectrum UV an IR spektrun can be
concluded that the compounds suspected isolated belonged to the Flanva-3-ol. Toxicity was conducted for prawn larvae
Artemia salina Leach. Effect of toxicity from isolate identified with presentage of prawn larva and counted by probit
analysis (LC 50 ). The results of this test showed that the the isolate was very toxic with LC 50 value of 26,8824 ppm. The
test result obtained mortality of shrimp larvae LC 50 value of each sample is 110.5176 ppm total extract, chloroform
fraction 147.7895 ppm, and isolate 80.2568 ppm.
Keywords : Terap (Artocarpus odoratissimus Blanco), Isolation, Flavonoid, BSLT
Downloads
References
Aliefman, H dan A. Wahab Jufri. 2011. Aktifitas Anti Malaria dan Analisis Metabolit Sekunder Kayu dan Kulit Batang Artocarpus odoratissimus blanco.FakultasKeguruandanIlmuPendidikanJurusan FMIPA: Universitas Mataram
Asih. A. 2009. Isolasi dan Identifikasi Senyawa Isoflavon Dari Kacang Kedelai (Glycine max). Bukit Jimbaran: Jurusan Kimia FMIPA UniversitasUdayana.
Harborne, J.B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: Penerbit ITB..
Jayasinghe L., Balasooriya BAIS, Padmini W C Hara N, and Fujimoto Y. 2004.Geranyl Chalcone Derivatives with Antifugal and Radical Scavenging.Phytochem 65: 1287-1290. [8] Cao, S., Butler, M. S., and Buss, A. D. 2003. Flavonoids from Artocarpus lanceifolius. Natural Product Research 17(2):79-81.
John, Coates. 2000.Interpretation of Infrared Spectra, A Practical Approach, in Encyclopedia of Analytical Chemistry, eds. J. Workman, A.W. Sprinsteen. New York : Academic Press
Khan M R, Omoloso Ad, Kihara M. 2003. Antibacterial activity of Artocarpus heterophyllus. Fitoterapia 74: 501-505
Ko H H, Lu Y H, Yang S Z, Won S J, and Lin C N. 2005. Cytotoxic Prenyl flavonoids from Artocarpus elasticus. J Nat Prod 68: 1692-1695.
Leny, S. 2006. “Isolasi dan Uji Bioaktifitas Kandungan Kimia Utama Puding Merah Dengan Metode Uji Brine Shirmp”.Karya Ilmiah Jurusan Kimia FMIPA, Universitas Sumatra Utara.
Verheij EWM, Coronel RE (eds). Plant Resources of South Asia No. 2 Edible Fruits and Nuts. Bogor:Prosea Foundation.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
This license requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, even for commercial purposes. If others remix, adapt, or build upon the material, they must license the modified material under identical terms.
BY: Credit must be given to you, the creator.
SA: Adaptations must be shared under the same terms.
