UJI FITOKIMIA, TOKSISITAS DAN AKTIVITAS ANTIBAKTERI EKSTRAK BERBAGAI FRAKSI DAUN MARA (Macaranga tanarius (L.) M.A) TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli
Abstract
Phytochemical, toxicity and antibacterial activity tests of total extract, fractions of n-heksana, ethyl acetate and ethanol-water from mara leaf (Macaranga tanarius (L.) M.A) from Samboja, East Kalimantan has been carried out. Based on the secondary metabolites phytochemical test of the (Macaranga tanarius (L.) M.A) leaf show that total extract is contain alkaloid, flavonoid, phenolic and steroid. n-hexane fraction is contain alkaloid, flavonoid and steroid. Ethyl acetate fraction is contain phenolic and triterpenoid. Ethanol-water fraction is contain alkaloid and phenolic. Brine shrimp lethality test values obtained Lethal Concentration 50 % (LC50) total extract 23.99 ppm , n-heksana fraction 13,93 ppm, ethyl acetate fraction 468,25 ppm and ethanol-water fraction 283,67 ppm. Antibacteria activity test of extracts for Staphylococcus aureus bacteria (positive Gram) and Escherichia coli (negative Gram) was carried out by paper disc method. The test showed that the most active were ethyl acetate fraction with minimum inhibitor consentration of 0,125- 0,5 % (b/v) which clear zone diameter was 7,25 mm on Staphylococcus aureus bacteria and 6,25 mm on Escherichia coli bacteria.
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References
Harborne, J.B. 1987. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Bandung: Penerbit ITB.
Kadarisman, I. 2000. Isolasi Dan Identifikasi Senyawa Kimia Bioaktif Dari Rimpang Bangle (Zingiber cassumunar Roxb). Skripsi Jurusan Kimia FMIPA, Institut Pertanian Bogor.
Kadarisman, I. 2000. Isolasi Dan Identifikasi Senyawa Kimia Bioaktif Dari Rimpang Bangle (Zingiber cassumunar Roxb). Skripsi Jurusan Kimia FMIPA, Institut Pertanian Bogor.
Lay, B.W dan Hastowo, S. 1992. Mikrobiologi. IPB : Bogor.
Lemmens, R.H.M.J. and Bunyapraphatsara, N. (Ed). 2003. Medicinal and Poisonous Plants 3. Plant resources of South – East Asia (Prosea). Prosea Foundation: Bogor.
Mahato, R. B and Chaudhary, R.P. 2005. Ethnomedicinal Study and Antibacterial Activities of Selected Plants Palpa District, Nepal. Department of Botany : Tribhuvan University. Scientific World. Vol 3 No 3.
Mayanti, T., Julaeha E. dan Putri A. 2010. Isolasi dan Karakterisasi Senyawa Antibakteri dari Fraksi Etil Asetat Kulit Batang Lansium Domesticum Corr. Cv Kokossan. Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Padjadjaran.
Noor, S.M., Masniari, P dan Titin, Y. 2006. Analisis Senyawa Kimia Sekunder dan Uji Daya Antibakteri Ekstrak Daun Tanjung (Mimusops Elengi L) terhadap Salmonella typhi dan Shigella boydii. Seminar Nasional Teknologi Peternakan dan Veteriner.
Nurhayati, A.P.D. 2006. Uji Toksisitas Ekstrak Eucheuma Alvarezii terhadap Artemia Salina sebagai Studi Pendahuluan Potensi Antikanker. Akta Kimindo Vol. 2.
Prasetyawan, A. 2011. Aktivitas antimikroba ekstrak etanol daun senggani (Melastoma affine D. Don) terhadap S. aureus, E. coli, dan C. albicans. Skripsi Tesis: Universitas Muhammadiyah: Surakarta.
Robinson, T. 1995. Kandungan Kimia Organik Tumbuhan Tingkat Tinggi. Bandung: Penerbit ITB.
Waluyo, L. 2005. Mikrobiologi Umum. Malang : Penerbit UMM Press.
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