Analisis Residu Klorpirifos Dalam Sayur-Sayuran Dengan Teknik High Performance Liquid Chromatography (HPLC)
The research about analysis of chlorpyrifos residue in vegetables by using High Performance Liquid Chromatography (HPLC) technique has been done. To obtain the optimal measurement results, the measurement performed several important parameters in the chromatographic system was composition of mobile phase, volume injection sample, flow rate and pH eluent. Optimum measurement conditions obtained was mobile phase composition (water : methanol) with 70 : 30, volume injection sample are 5 mL, flow rate are 0.5mL/menit and pH eluent are 7. The analytical performance that obtained is good showed with the reproducibility value as percentage coefficient variance (% CV) was 0.0664%, limit of detection (LOD) was 0.44 ppm, with a recovery percentage of > 95%. The results obtained showed the HPLC technique can be used for the routine analysis in the determination of chlorpyrifos for the vegetable samples.
Erin, M. B, Hertz-Picciotto, I, and Beaumont, J.J. 2001. Fetal Deaths Linked to Living Close to Agricultural Pesticide Use During Weeks 3-8 of Pregnancy, Epidemiology. 52. pp. 105 – 109.
Schreinemachers, D. M. 2003. Birth Defects Higher in Babies Born to Families Living Near Farming Areas Using Pesticides, Environmental Health Perspectives. 111(9). pp. 1259-1264.
Sastroutomo, S.S. 1992. Pestisida: Dasar Dasar dan Dampak Penggunaannya, Jakarta: Gramedia Pustaka Utama.
Islam, S, Afrin, N, Hossain, M.S, Nahar, N, Mosihuzzaman, M dan Mamun, M.I.R. 2009. Application of High Performance Liquid Chromatography to the Analysis of Pesticide Residues in Eggplants. J. Applied Sci. pp. 325-329.
Panggabean, A. S., Amran, M.B., Buchari and Achmad, S. 2009. Speciation of Organotin Compounds with Ion Pair-Reversed Phase Chromatography Technique. Eurasian Journal of Analytical Chemistry. 4(2). pp 215-225.
Miller. J.C dan J. N Miller, 1991. Statistika Untuk Kimia Analitik. Edisi Kedua. Penerbit ITB. Bandung.
Panggabean, A.S., Subur P. Pasaribu, Bohari, Nurhasanah 2014. Preconcentration of Chromium(VI) at Trace Levels Using Acid Alumina Resin With Column Method Indo. J. of Chem. 14(1). pp. 51-56
Snyder, L.R., Kirkland, J.J., and Glajch, J.L. 1997. Practical HPLC Method Development. 2nd Edition. John Wiley & Sons, Inc. New York.
Hayun, H. Y dan Citra N. A. 2004. Penetapan Kadar sakarin, Asam Benzoat, Asam Sorbat, Kofeina, dan Aspartam didalam beberapa Minuman Ringan Bersoda secara Kromatografi Cair Kinerja Tinggi. Majalah Ilmu Kefarmasian,Vol I, No 3 Hal 148-159.
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