Rice Husk Silica-Coated Magnetic Particles As A Low Cost Support Material For Crude Pseudomonas Aeruginosa Lipase Immobilization

  • Noor Hindryawati Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Abstract

In this research, lipase produced from the bacteria Pseudomonas aeruginosa was immobilized on rice husk silica-coated Fe3O4 nanoparticles. The process included the following steps: preparation of magnetic ferrosoferric oxide nanoparticles (NP), coating NP with silica from rice husk ash, activation with glutaraldehyde and covalent immobilization of lipase on the support. The synthesis of the nanoparticle was followed by characterization through FT-IR, XRD, and FE-SEM. The hydrolysis kinetics (using 4-Nitrophenyl palmitate as a substrate) of the immobilized lipase followed Michaelis-Menten model with a Vmax and a Km value of 4.0 mM.s-1 and 0.63 mM, respectively. The immobilized lipase showed better tolerance to extreme temperature and pH compared to free lipase. About 60% of enzyme remained immobilized after ten cycles of reuse and 68.13% of it were stable until 49 days. Thus the silica-coated Fe3O4 nanoparticles appeared to be a potential support material for lipase immobilization applications.

Keyword
Core-shell magnetic nanoparticles, Pseudomonas aeruginosa, immobilized lipase, rice-husk silica

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References

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Published
2016-11-08
How to Cite
HINDRYAWATI, Noor. Rice Husk Silica-Coated Magnetic Particles As A Low Cost Support Material For Crude Pseudomonas Aeruginosa Lipase Immobilization. JURNAL KIMIA MULAWARMAN, [S.l.], v. 14, n. 1, nov. 2016. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/376>. Date accessed: 24 apr. 2024.
Citation Formats
Issue
Vol 14 No 1 (2016)
Section
Artikel

Keywords

Core-shell magnetic nanoparticles; Pseudomonas aeruginosa; immobilized lipase; rice-husk silica.

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