Rice Husk Silica-Coated Magnetic Particles As A Low Cost Support Material For Crude Pseudomonas Aeruginosa Lipase Immobilization

  • Noor Hindryawati Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Abstract

In this research, lipase produced from the bacteria Pseudomonas aeruginosa was immobilized on rice husk silica-coated Fe3O4 nanoparticles. The process included the following steps: preparation of magnetic ferrosoferric oxide nanoparticles (NP), coating NP with silica from rice husk ash, activation with glutaraldehyde and covalent immobilization of lipase on the support. The synthesis of the nanoparticle was followed by characterization through FT-IR, XRD, and FE-SEM. The hydrolysis kinetics (using 4-Nitrophenyl palmitate as a substrate) of the immobilized lipase followed Michaelis-Menten model with a Vmax and a Km value of 4.0 mM.s-1 and 0.63 mM, respectively. The immobilized lipase showed better tolerance to extreme temperature and pH compared to free lipase. About 60% of enzyme remained immobilized after ten cycles of reuse and 68.13% of it were stable until 49 days. Thus the silica-coated Fe3O4 nanoparticles appeared to be a potential support material for lipase immobilization applications.

Keyword
Core-shell magnetic nanoparticles, Pseudomonas aeruginosa, immobilized lipase, rice-husk silica

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References

[1] Sangeetha R, Arulpandi I, Geetha A. Bacterial Lipases as Potential Industrial Biocatalysts: An Overview. Research J. Microbiol., 6, 1-24(2011).
[2] Lorenzo M D. Improvement of lipase stability in the presence of commercial triglycerides. Ph. D. Thesis, Università di Napoli Federico II., Italy (2007).
[3] Yapasan E. Partial purification and characterization of lipase enzyme from a Pseudomonas strain. Master Thesis, Izmir Institute of Technology., Turkey (2008).
[4] Mikkelsen, Corton. Bioanalytical Chemistry, Jhon Wiley & Sons,Inc, (2004).
[5] Anming W, Cheng Z, Hua W, Shubao, S, Jianyue X, Pingkai, Q., Covalent assembly of penicillin acylase in mesoporous silica based on macromolecular crowding theory. Chin. J. Chem. Eng., 15(6), 788-790 (2012).
[6] Ranjbakhsh E, Bordbar AK, Abbasi M, Khosropour AR, Shams E. Enhancement of stability and catalytic activity of immobilized lipase on silica-coated modified magnetite nanoparticles. Chem. Eng. J., 179, 272-276 (2012).
[7] Anming W, Hua W, Cheng Z, Zhiqiang D,
Shemia Z, Shubao, S. Ag-induced efficient immobilization of papain on silica spheres. Chin. J. Chem. Eng., 16(6), 788-790 (2007).
[8] Wang X, Dou P, Zhao P, Zhao C, Ding Y, Xu P. Immobilization of lipases onto magnetic Fe3O4 nanoparticles for application in biodiesel production. ChemSusChem., 2, 947-950 (2009).
[9] Xie W, Ma N. Immobilized lipase on Fe3O4 nanoparticles as biocatalys for biodiesel production. Energy & Fuel, 23(3), 1347–1353 (2009).
[10] Sun Y, Duan L, Guo Z, Duan M Y, Ma M, Xu L, Zhang Y, Gu N. An improved way to prepare superparamagnetic magnetite-silica core-shell nanoparticles for possible biological application. J.Magn. Magn. Mater., 285, 65-70 (2005).
[11] He F, Zhuo R X, Liu L J, Jin D B, Feng J, Wang X L. Immobilization lipase on porous silica beads: preparation and application for enzymatic ring-opening polymerization of cyclic phosphate. React. Funct. Polym., 47, 153-158 (2001).
[12] Lee D H, Park C H, Yeo J M, Kim S W. Lipase immobilization on silica gel using cross-linking method. J. Ind. Eng., 13(5), 777-782 (2006).
[13] Zeng L, Luo K, Gong Y. Preparation and characterization of dendritic composite magnetic particles as a novel enzyme immobilization carrier. J. Mol. Catal. B: Enzym., 38, 24-30 (2006).
[14] Andrade A L, Souza D M, Pereira M C, Fabris J D, Domingues R Z. Synthesis and Characterization of magnetic nanoparticles coated with silica through a sol-gel approach. Ceramica. 55, 420-424 (2009).
[15] Tran D, Chen C, Chang J. Immobilization of Burkholderia sp. lipase on a ferric silica nanocomposite for biodiesel production. J. Biotechnol., 158, 112-119 (2012).
[16] Ranganath K V S, Glorius F. Superparamagnetic nanoparticles for asymmetric catalysis-a perfect match. Catal. Sci. Technol., 1, 13-22(2011).
[17] Lei L, Liu X, Li Y, Cui Y, Yang Y, Qin G Study on synthesis of poly(GMA)-grafted Fe3O4/SiOx magnetic nanoparticles using atom transfer radical polymerization and their application for lipase immobilization. Mater. Chem. Physics., 125, 866-871(2011).
[18] Fajari M, Yamini Y, Rezaee M. Magnetic nanoparticles: synthesis, stabilization, functionalization, characterization, and applications. J. Iran. Chem. Soc., 7(1), 1-37(2010).
[19] Huacong Z, Wei L, Qinghui S, Hongshuai G, Peng X, Fuli D, Huizhou L. Immobilization of pinicilin G acylase on magnetic nanoparticles modified by ionic liquids. Chin. J. Chem. Eng., 20(1), 146-151 (2012).
[20] Lien Y, Wu T. Preparation and characterization of thermosensitive polymers grafted onto silica-coated iron oxide nanoparticles. J Colloid Interf. Sci., 326, 517-521(2008).
[21] Chen G, Kuo C, Chen C, Yu C, Hieh C, Liu Y. Effect of membranes with various hydrophobic/hydrophilic properties on lipase immobilized activity and stability. J. Biosci. Bioeng., 113, 166-172(2012).
[22] Chaubey A, Parshad R, Taneja S C, Qazi G N. Arthrobacter sp. lipase immobilization on magnetic sol–gel composite supports for enantioselectivity improvement. Process Biochem., 44, 154-160(2009).
[23] Yang J, Ma X, Zhang Z, Chen B, Li S, Wang G. Lipase Immobilized by Modification-Coupled and Adsorption-Cross-Linking Methods: A Comparative Study. Biotechnol. Adv., 28(5), 644-650(2010).
Published
2016-11-08
How to Cite
HINDRYAWATI, Noor. Rice Husk Silica-Coated Magnetic Particles As A Low Cost Support Material For Crude Pseudomonas Aeruginosa Lipase Immobilization. JURNAL KIMIA MULAWARMAN, [S.l.], v. 14, n. 1, nov. 2016. ISSN 2476-9258. Available at: <https://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/376>. Date accessed: 11 may 2025.
Citation Formats
Issue
Vol 14 No 1 (2016)
Section
Artikel

Keywords

Core-shell magnetic nanoparticles; Pseudomonas aeruginosa; immobilized lipase; rice-husk silica.

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