ANALISIS FITOKIMIA DAN TOKSISITAS (BRINE SHRIMP LETHALITY TEST) EKSTRAK SERBUK SARI DARI Trigona incisa

  • Novalien Fiergiyanti Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
  • Erwin - Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
  • Syafrizal - Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Abstract

The phytochemical, brine shrimp lethality and activity antioxidant test and secondary metabolits of bee pollen have been conducted. The pollen samples are extract with solvent ethanol by masseration method and that is concentrated by using rotary evaporator. The ethanol crude extract obtained are fractioned with solvent ethanol and solvent ethyl acetat. Based on secondary metabolits phytochemical test of bee pollen showed that ethanol crude extract contains alkaloid and fenolik. Ethanol fraction extract contains alkaloid. Ethyl acetat fraction extract contains alkaloid and fenolik. In brine shrimp lethality test, the increase larvae (Artemia salina L.) death and data was yielded recorded then processed using SAS Probit Analysis to determine the lethal concentration 50% (LC50) value. The results of this test obtained data and showed that the most active fraction is ethanol fraction extract with value of LC50 was equel to 249,60 ppm.
Keywords: Bee pollen, phytochemical test, brine shrimp lethality test (BSLT).

Downloads

Download data is not yet available.

References

Achmad, S. A. 1986. Kimia Organik Bahan Alam. Jakarta: Karunika.

Faegri, K. 1989. Text Book Pollen Analysis Edition IV . London. Alden Press.

Harborne, J.B. 1987. Metode Fitokimia. Bandung: Penerbit ITB.

Mclaughlin. 1998. The Use of Biological Assays to Evaluate Botanicals. Drug Information Journal. Vol 32: 513-524.

Meyer, B. N, N.R. Ferrigni, J.E. Putman, L.B. Jacobsen, D.E. Nichol dan J.L. Melaughlin. 1982. Brine Shrimp: A Vonvenient General Bioassay for Avtive Plant Constituents. Planta Medica 45:31-34.

Sholikhah, M. 2012. Analisis Fitokimia dan Uji Daya Antimikroba Ekstrak Produk Sarang Lebah Trigona incisa Terhadap Streptococcus sobinus dan Candida albicans. Skripsi Fakultas Matematika dan Ilmu Pengetahuan Alam :UNMUL
Published
2016-01-19
How to Cite
FIERGIYANTI, Novalien; -, Erwin; -, Syafrizal. ANALISIS FITOKIMIA DAN TOKSISITAS (BRINE SHRIMP LETHALITY TEST) EKSTRAK SERBUK SARI DARI Trigona incisa. JURNAL KIMIA MULAWARMAN, [S.l.], v. 13, n. 1, jan. 2016. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/42>. Date accessed: 26 apr. 2024.
Section
Artikel

Most read articles by the same author(s)

1 2 > >>