ANALISIS FITOKIMIA DAN TOKSISITAS (BRINE SHRIMP LETHALITY TEST) EKSTRAK SERBUK SARI DARI Trigona incisa
Abstract
The phytochemical, brine shrimp lethality and activity antioxidant test and secondary metabolits of bee pollen have been conducted. The pollen samples are extract with solvent ethanol by masseration method and that is concentrated by using rotary evaporator. The ethanol crude extract obtained are fractioned with solvent ethanol and solvent ethyl acetat. Based on secondary metabolits phytochemical test of bee pollen showed that ethanol crude extract contains alkaloid and fenolik. Ethanol fraction extract contains alkaloid. Ethyl acetat fraction extract contains alkaloid and fenolik. In brine shrimp lethality test, the increase larvae (Artemia salina L.) death and data was yielded recorded then processed using SAS Probit Analysis to determine the lethal concentration 50% (LC50) value. The results of this test obtained data and showed that the most active fraction is ethanol fraction extract with value of LC50 was equel to 249,60 ppm.Keywords: Bee pollen, phytochemical test, brine shrimp lethality test (BSLT).
Downloads
References
Faegri, K. 1989. Text Book Pollen Analysis Edition IV . London. Alden Press.
Harborne, J.B. 1987. Metode Fitokimia. Bandung: Penerbit ITB.
Mclaughlin. 1998. The Use of Biological Assays to Evaluate Botanicals. Drug Information Journal. Vol 32: 513-524.
Meyer, B. N, N.R. Ferrigni, J.E. Putman, L.B. Jacobsen, D.E. Nichol dan J.L. Melaughlin. 1982. Brine Shrimp: A Vonvenient General Bioassay for Avtive Plant Constituents. Planta Medica 45:31-34.
Sholikhah, M. 2012. Analisis Fitokimia dan Uji Daya Antimikroba Ekstrak Produk Sarang Lebah Trigona incisa Terhadap Streptococcus sobinus dan Candida albicans. Skripsi Fakultas Matematika dan Ilmu Pengetahuan Alam :UNMUL
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
This license requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, even for commercial purposes. If others remix, adapt, or build upon the material, they must license the modified material under identical terms.