Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.)

  • Sari Setianingsih Jurusan Kimia FMIPA UNMUL
  • Rudi Kartika Jurusan Kimia FMIPA Universitas Mulawarman
  • Partomuan Simanjuntak Puslit Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
DOI: https://doi.org/10.30872/jkm.v15i1.491

Abstract

This study was started by extraction of Eucalyptus deglupta Blume. Using organic solvent   (n-hexane, ethyl acetate, ethanol and water) followed by phytochemical screening and toxicity test using Brine Shrimp Lethality Test (BSLT) method. Isolation and identification of chemical compounds contained in the fraction were done by column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids and phenolics in the extract. Toxicity test results showed that the ethyl acetate extract was potentially active with LC50 value of  617.95 ppm. The extract was continued to isolation stage and gave fraction EKEA-3.1 with LC50 value of 2759.93 ppm. Identification of chemical compounds in EKEA-3.1 with KG-MS analysis showed that EKEA-3.1 was suspected to be Stigmastan-3,5-diene.

Keyword
Eucalyptus deglupta Blume., Myrtaceae, Brine Shrimp Lethality Test (BSLT), stigmastan-3.5-diene

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References

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Published
2017-11-29
How to Cite
SETIANINGSIH, Sari; KARTIKA, Rudi; SIMANJUNTAK, Partomuan. Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.). JURNAL KIMIA MULAWARMAN, [S.l.], v. 15, n. 1, p. 1-4, nov. 2017. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/491>. Date accessed: 26 apr. 2024. doi: https://doi.org/10.30872/jkm.v15i1.491.
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Vol 15 No 1 (2017)
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