• Binawati Ginting Jurusan Kimia, Fakultas MIPA, Universitas Syiah Kuala, Banda Aceh
  • Siti Hadijah Jurusan Kimia, Fakultas MIPA, Universitas Syiah Kuala, Banda Aceh
  • Hira Helwati Jurusan Kimia, Fakultas MIPA, Universitas Syiah Kuala, Banda Aceh


The isolation of antioxidant active compounds from n-hexane extract of nutmeg leaves (Myristica fragrans Houtt) with dipphenylpicrylhydrazyl (DPPH) has been carried out. n-Hexane extract of nutmeg leaves (0.35%) was obtained by partitioning of methanol extract (535.7 g) using n-hexane solvent. Isolation of active compound of n-hexane extract with gravitational column chromatography gradient elution of n-hexane : ethyl acetate with ratio (9 : 1) – (7 : 3) obtained 7 mixed fraction of MFHD1 - MFHD 7. MFHD 2 fraction further isolated obtained MFHD 2.1. The nutmeg n-hexane extracts and their fractions showed strong antioxidant activity with IC50 values ​​<100 ppm except MFHD 6 fraction showed for IC50 values of 114.95 ppm. The most potent antioxidant activity in MFHD 4 fraction with IC50 value of 0.729 ppm (compared with vitamin C value of IC50 7,875 ppm as positive control). The GC-MS analysis of the MFHA 2.1 fraction shows the presence of sitostenone and eucalyptol compounds, both of which have been reported to have been isolated from several different plants and have antioxidant activity.


[1] Putra, PD, Verawati, 2011, Analisa Kandungan Flavonoid dan Aktivitas Antioksidan dari Rempah Tumbuhan Obat Sumatra Barat, Scientia Vol.1 No.1, Februari 2011, ISSN : 2087-5045
[2] Winarsi H, 2007, Antioksidan Alami & Radikal Bebas Potensi dan Aplikasinya Dalam Kesehatan, Penerbit Kanisius (Anggota IKAPI), Yogyakarta
[3] Nagja, T, Vimal, K, Sanjeev, A, 2016, Myristica fragrans : A Comprehensive Review, International Journal of Pharmacy and Pharmaceutical Science, ISSN-0975-1491.
[4] Nurmilasari, Ginting, B, Helwati, H, 2017, Isolation of Antioxidant Compounds of Metanol Extract of Nutmeg Leaves (Mirystica fragrans Houtt), Jurnal Natural Vol.1, 2017, pISSN 1411-8513, eISSN 2541-4060.
[5] Ginting, B, Mustanir, Hira H., Lidya S.D, Eralisa, Mujahid, R, 2017, Antioxidant activity of Hexane Extract Of Nutmeg Plants From South Aceh Province, Jurnal Natural Vol.1,
[6] Molyneux, P, 2014, The Use of The Stable Free Radical Dipphenylpicrylhydrazyl (DPPH) For Estimating Antioxidant Activity, Original Article, Songklanakarin J, Sci, Technol,. 204, 26(2) : 211-219.
[7] Hui, L, He, L, Huan, L, XiaoLan, L, AiGuo, Z, 2010, Chemical Composition of Lavender Essential Oil its Antioxidant Activity and Inhibition Against Rhinitis-related Bacteria, African Journal of Microbilogy research Vol. 4(4), ISSN 1996-0808 2010 Academic Journals.
[8] Bayala, B, Bassole, NHI, Gnoula, C, Nebie, R, Yonli, A, Morel, L, Figueredo, G, Nikiema, BJ, Lobaccari, AMJ, Simpore, J, 2014, Chemical Composition, Antioxidant, Anti-Inflammatory and Anti-Proliferative Activities of Essential Oils Of Plants From Burkina Faso, Open Access freely available online, Plos One
[9] Solano, R, L, Breksa, PA, Torres, V, B, J, Escalante, A, M, Herwedia, BJ, 2017, Chemical Composition and Antioxidant Activity of Lippia alba Essential Oil Obtained by Supercritical CO2 and Hydrodistillation, African Journal of Biotechnology, Vol. 16(17), ISSN. 1684-5315.
[11] Li, TC, kao, LC, Li, TH, Huang, TS, Chen, YC, 2015, Secondary Metabolites From The Leaves of Cinnamomum macrostemon Hayata, Reasearc article, European Journal Of biomedical And Pharmaceutical Sciences, ISSN 2349-8870.
[10] Liu, MC, Kao, LC, Wu, MH, Li, JW, Huang, TC, Li, TH, Chen, YC, 2014, Antioxidant and Anticancer Aporphine Alkaloids From The Leaves of Nelumbo nucifera Gaertn. Cv. Rosa-plena, Molecules, ISSN 1420-3049.
How to Cite
GINTING, Binawati; HADIJAH, Siti; HELWATI, Hira. ISOLATION OF ANTIOXIDANT COMPOUNDS OF N-HEXANE EXTRACT OF NUTMEG (Myristica fragrans Houtt) LEAVES. JURNAL KIMIA MULAWARMAN, [S.l.], v. 15, n. 1, p. 46-52, nov. 2017. ISSN 2476-9258. Available at: <>. Date accessed: 16 jan. 2018.