ISOLATION OF STEROID COMPOUNDS IN THE n-HEKSANA FRACTION FROM JARUM TUJUH BILAH LEAVES EXTRACT (Leuenbergeria bleo (Kunth) D.C.)

  • Yuli Yana Jurusan Kimia FMIPA Universitas Mulawarman
  • Chairul Saleh Jurusan Kimia FMIPA Universitas Mulawarman
  • Noor Hindryawati Jurusan Kimia FMIPA Universitas Mulawarman

Abstract

This study aims to find secondary metabolites isolated from the leaves of the Jarum Tujuh Bilah (Leuenbergeria bleo (Kunth) D.C.). The method used was maceration, fractionation and column chromatography and was identified using Fourier Transformra Infra Red (FT-IR). The phytochemical preliminary test of the n-hexane fraction contains the steroid secondary metabolite compound. The results of column chromatography produced 75 vial bottles which were then identified by thin layer chromatography and produced 8 combined fractions (A-H fraction) based on the similarity of characterization, from the 8 fractions D and E forming yellow crystals. The yellow crystals formed are then cleaned and recrystallized to produce 11.7 mg white amorphous crystals. Compounds are characterized by FTIR spectrometers. Based on FTIR analysis, isolates showed IR spectrum λ max cm-1: 3375; 2927,98; 2852,18; 1680; 1462,51; 1376.81; 1329.74; 1043,92; 1022.02 and 956.27. Based on the results obtained by isolates, it is suspected that the compound is a steroid sterol.

Keyword
Secondary metabolites, Isolation and fractionation, n-hexane fraction leave of Jarum Tujuh Bilah Leuenbergeria bleo (Kunth) D.C.), steroid sterol

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References

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Published
2019-11-29
How to Cite
YANA, Yuli; SALEH, Chairul; HINDRYAWATI, Noor. ISOLATION OF STEROID COMPOUNDS IN THE n-HEKSANA FRACTION FROM JARUM TUJUH BILAH LEAVES EXTRACT (Leuenbergeria bleo (Kunth) D.C.). JURNAL KIMIA MULAWARMAN, [S.l.], v. 17, n. 1, p. 31-36, nov. 2019. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/904>. Date accessed: 25 nov. 2020. doi: https://doi.org/10.30872/jkm.v17i1.904.
Section
Artikel