ISOLASI DAN KARAKTERISASI LIPASE DARI KECAMBAH BIJI ALPUKAT (Persea americana Mill)

Nurillah Sya'bani; Winni Astuti; Djihan Ryn Pratiwi

Nurillah Sya'bani Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Winni Astuti Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Djihan Ryn Pratiwi Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman

Abstract

Isolation and determination of optimum lipase conditions from Avocado seeds germination (Persea americana Mill) have been done. The purpose of this study is to determine the optimum working conditions and lipase specific activity of avocado seeds germination. Lipase was isolated using homogenization and centrifugation techniques. The crude extract of the enzyme containing the lipase produced from avocado seeds germination was used for the determination of total protein concentration and tested its activity on the pH conditions, temperature, and optimum substrate concentration with different with various variations. Determination of total concentration was done by Bradford method, while the determination of optimum working conditions and lipase specific activity was performed by titrimetric method. The results showed that total protein concentration in avocado seeds germination was 458 μg / mL. The optimum working conditions on avocado seed sprouts are the degree of acidity (pH) 6, temperature 35 ^oC, and 1.5% v/v substrate concentration. The lipase specific activity of avocado seeds germination is 144,1 U / mg.

Keyword

Lipase, avocado seeds germination (Persea americana Mill), lipase specific activity

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References

[1] Champe, C.P., Harvey, A.R., Ferrier, R., dan Denise. 2010. Biokimia Ulasan Bergambar Edisi 3. Jakarta: EGC.
[2] Sana, N.K., Hosin, Haque, and Ranajit, K.S. 2004. Identification, Purification, and Characterization of Lipase from Germinating Oil Seeds (Brassica napus L.). Bangladesh: University of Rajsahi.
[3] Arifan, F., Yulianto, M.E., Wikanta, D.K., dan Damayanti, N. 2011. Pengembangan Bioreaktor Enzimatik Untuk Produksi Asam Lemak dari Hasil Samping Penggilingan Padi Secara In Situ. Semarang: Jurusan Teknik Kimia PSD III UNDIP.
[4] Prasetyowati. 2010. Pengambilan Minyak Biji Alpukat (Persea Americana Mill) dengan Metode Ektsraksi. Universitas Sriwijaya.
[5] Endy, M., Nanik, D., Fiqih, P.J., dan Hermawan, D.A. 2010. Pengembangan Proses Enzimatis untuk Produksi Asam Lemak secara In Situ. Semarang: UNDIP.
[6] Puspitaningrum, R., Chris, A., dan Biomed, M. 2016. Enzim dan Pemanfaatannya. Bogor: Ghalia Indonesia.
[7] Amalia, R., Rumondang, B., dan Firman, S. 2013. Penentuan pH dan Suhu Optimum untuk Aktivitas Ekstrak Kasar Enzim Lipase dari Kecambah Biji Karet (Hevea Brasiliensis) terhadap Hidrolisis PKO (Palm Kernel Oil). Medan: Universitas Sumatera Utara.
[8] Poedjiadi, A. 1994. Dasar-dasar Biokimia. Jakarta: UI-Press.
[9] Sadikin, M. 2002. Biokimia Enzim. Jakarta: Widya Medika.
[10] Su’i, M., Harijono, Yunianta, dan Aulani’am. 2010. Aktifitas Hidrolisis Enzim Lipase dari Kentos Kelapa terhadap Minyak Kelapa. Malang: Universitas Brawijaya.