SKRINING LIPASE DARI ISOLAT BAKTERI ENDOFIT BATANG PACING (Costus speciosus (J.Koenig) Sm) DAN PENENTUAN KONDISI KERJA OPTIMUMNYA

  • Asjayani Kurnia Sari Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Winni Astuti Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University
  • Djihan Ryn Pratiwi Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University

Abstract

Endophytic bacteria are bacteria that live in plant tissues such as the roots, leaves, and stems of plants. This research was conducted to screen lipase-producing endophytic bacteria from Pacing stem (Costus speciosus (J.Koenig) Sm.) and determine the optimum working conditions. Screening of lipase-producing bacteria on nutrient agar media containing olive oil and Rhodamine B showed that one isolate was able to produce lipase. The crude lipase extract produced from endophytic bacteria worked optimally at a pH of 7, the temperature of 40℃, and a substrate concentration of 2%.


 


Keywords : Costus speciosus (J.Koenig) Sm., Endophytic bacteria, Lipase, Optimum Activity


 

Keyword
Costus speciosus (J.Koenig) Sm., Endophytic bacteria, Lipase, Optimum Activity

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Published
2020-09-28
How to Cite
SARI, Asjayani Kurnia; ASTUTI, Winni; PRATIWI, Djihan Ryn. SKRINING LIPASE DARI ISOLAT BAKTERI ENDOFIT BATANG PACING (Costus speciosus (J.Koenig) Sm) DAN PENENTUAN KONDISI KERJA OPTIMUMNYA. JURNAL ATOMIK, [S.l.], v. 5, n. 1, p. 1-5, sep. 2020. ISSN 2549-0052. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JA/article/view/689>. Date accessed: 31 oct. 2020.
Section
Artikel