ISOLASI BAKTERI ENDOFIT DARI BUAH OKRA (Abelmoschus esculentus L. Moench)

Adha Mikdariawan; Winni Astuti; Eva Marliana

Adha Mikdariawan Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Winni Astuti Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman
Eva Marliana Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman

Abstract

Endophytic bacteria is a bacteria that live in plant tissues without disrupt the plant's life cycle. This research aims to isolate endophytic bacteria from okra fruit (Abelmoschus esculentus L. Moench). Before isolation, okra fruit was sterilized by the outer surface with aquadest, 5.25% NaClO and 95% ethanol. The okra fruit sample is then cut and the inside of the okra fruit is cultured in a nutrient agar (NA). The results of this test indicate that there are endophytic bacteria that grow characterized by the presence of turbid areas around the sample. The results of isolation of endophytic bacteria using the method of stocking cup were obtained by 28 isolates of endophytic bacteria.

Keyword

Endophytic bacteria, Abelmoschus esculentus L. Moench

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References

[1] Simarmata R., Lekatompessy S. dan Sukiman, H. 2007. Isolasi mikroba endofitik dari tanaman obat Sambung Nyawa (Gymura procumbens) dan analisis potensinya sebagai antimikroba. Berk Penel Hayati, 13:85-90.
[2] Desriani, Safira U. M., Bintang M., Rivai A. dan Lisdiyanti P. 2014. Isolasi dan karakterisasi bakteri endofit dari tanaman binahong dan ketapang china. Jurnal Kesehatan Andalas, 3(2):89-93.
[3] Sagita D., Suharti N., dan Azizah N. 2017. Isolasi bakteri endofit dari daun sirih (Piper betle L.) sebagai antibakteri terhadap Escherichia coli dan Staphylococcus aureus. Jurnal Ipteks Terapan, 11(1): 65-74.
[4] Pujiyanto S., Sunarno, dan Widyasari A. 2015. Isolasi dan karakterisasi bakteri endofit penghasil inhibitor α-Glukosidase dari tanaman pare (Momordica Charantia L.). Prosiding SNST.