Uji Aktivitas Penghambatan Enzim α-Amilase dan Glukoamilase dari Ekstrak Etanol Daun Kirinyuh (Chromolaena odorata L.)
Abstract
Research has been carried out to test the inhibitory activity of α-amylase and glucoamylase enzymes in the ethanolic extract of kirinyuh (Chromolaena odorata L.) leaves. The use of kirinyuh leaf ethanol extract as a diabetes drug in vivo has been carried out, but its mechanism of action have never been researched before, so further research is needed on the specific mechanism of ethanolic extract of kirinyuh leaves in reducing blood sugar levels, especially through inhibition of α-amylase and glucoamylase enzymes in vitro. The purpose of this study was to test the antidiabetic activity of the ethanol extract of kirinyuh leaves in vitro using α-amylase and glucoamylase inhibition methods. This research uses DNS spectrophotometric method. The results showed that kirinyuh leaf ethanol extract proved to have inhibitory activity against α-amylase and glucoamylase enzymes with IC50 values of 3730.15 ± 28.91 ppm or 3730.15 ± 28.91 µg/mL and 2510.78. ± 383.37 ppm or 2510.78 ± 383.37 µg/mL. From the results, it can be concluded that the ethanolic extract of kirinyuh leaves has inhibitory activity against α-amylase and glucoamylase enzymes.
Telah dilakukan penelitian uji aktivitas penghambatan enzim α-amilase dan glukoamilase ekstrak etanol daun kirinyuh (Chromolaena odorata L.). Penggunaan ekstrak etanol daun kirinyuh sebagai obat diabetes secara in vivo telah dilakukan,tetapi belum diketahui mekanisme kerjanya, sehingga perlu dilakukan penelitian lebih lanjut mengenai mekanisme spesifik ekstrak etanol daun kirinyuh pada penurunana kadar gula darah terutama melalui aktivitas penghambatan terhadap enzim α-amilase dan glukoamilase secara in-vitro. Tujuan penelitian ini untuk untuk menguji aktivitas antidiabetes ekstrak etanol daun kirinyuh secara in vitro menggunakan metode penghambatan enzim α-amilase dan glukoamilase. Penelitian ini menggunakan metode DNS secara spektrofotometri. Hasil penelitian menunjukkan ekstral etanol daun kirinyuh terbukti memiliki aktivitas penghambatan terhadap enzim α-amilase dan glukoamilase dengan nilai IC50 masing-masing, yaitu sebesar 3730,15 ± 28,91 ppm atau 3730,15 ± 28,91 μg/mL dan 2510,78 ± 383,37 ppm atau 2510,78 ± 383,37 μg/mL. Dari hasil dapat disimpulkan bahwa ekstrak etanol daun kirinyuh memiliki aktivitas penghambatan terhadap enzim α-amilase dan glukoamilase.
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References
[2] International Diabetes Federation. (2019). IDF Diabetes Atlas. IDF Diabetes Atlas. https://diabetesatlas.org/atlas/ninth-edition/
[3] Kemenkes RI. (2018). Laporan Hasil Riset Kesehatan Dasar (Riskesdas) Badan Penelitian dan Pengembangan Kesehatan. Badan LITBANGKES Kementerian Kesehatan RI. https://www.litbang.kemkes.go.id/laporan-riset-kesehatan-dasar-riskesdas/
[4] Khoiri, W. H. (2021). Efek Penyembuhan Ekstrak Daun Kirinyuh (Chromolaena odorata) terhadap Luka Diabetes pada Tikus Putih (Rattus novergicus). Universitas Perintis Indonesia.
[5] Yenti, R., Afrianti, R., & Afriani, L. (2011). Formulasi Krim Ekstrak Etanol Daun Kirinyuh (Euphatorium odoratum. L) untuk Penyembuhan Luka. Artikel PharmaMedika, 3(1), 227–230. https://doi.org/10.33476/MKP.V3I1.440
[6] Barku, V. Y. A., Boye, A., & Ayaba, S. (2013). Phytochemical Screening and Assessment of Wound Healing Activity of the Leaves of Anogeissus leiocarpus. European Journal of Experimental Biology, 3(4), 18–25.
[7] Wirasti, W., Lestari, T., & Isyti’aroh, I. (2021). Penghambatan Ekstrak Daun Kremah (Alternanthera sessilis) terhadap Enzim α-Amilase secara In-Vitro. Pharmacon: Jurnal Farmasi Indonesia, 18(1), 68–74.
[8] Sugiwati, S., Setiasih, S., & Afifah, E. (2009). Antihyperglycemic Activity of the Mahkota Dewa Leaf Extracts as an Alpha-Glucosidase Inhibitor. Makara Journal of Health Research, 13(2), 74–78.
[9] Wicaksono, I. B., & Ulfah, M. (2017). Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Daun Sirsak (Annona muricata L.) dan Daun Jambu Biji (Psidium guajava L.) dengan Metode DPPH (2,2-Difenil-1- Pikrilhidrazil). Inovasi Teknik Kimia, 2(1), 44–48.
[10] Khairina, A., & Leny, Y. (2015). Pengaruh Variasi Lama Penyimpanan Umbi Bengkuang (Pachirhyzus erozus) terhadap Kadar Glukosa Darah Rattus norvegicus. Unesa, Journal of Chemistry, 4(1), 31–36.
[1] Dipiro, J. T., Talbert, R. L., Yee, G. C., Matzke, G. R., Wells, B. G., & Posey, L. M. (2017). Pharmacotherapy: A Pathophysiologic Approach (10th ed.). McGraw-Hill Education.
[2] International Diabetes Federation. (2019). IDF Diabetes Atlas. IDF Diabetes Atlas. https://diabetesatlas.org/atlas/ninth-edition/
[3] Kemenkes RI. (2018). Laporan Hasil Riset Kesehatan Dasar (Riskesdas) Badan Penelitian dan Pengembangan Kesehatan. Badan LITBANGKES Kementerian Kesehatan RI. https://www.litbang.kemkes.go.id/laporan-riset-kesehatan-dasar-riskesdas/
[4] Khoiri, W. H. (2021). Efek Penyembuhan Ekstrak Daun Kirinyuh (Chromolaena odorata) terhadap Luka Diabetes pada Tikus Putih (Rattus novergicus). Universitas Perintis Indonesia.
[5] Yenti, R., Afrianti, R., & Afriani, L. (2011). Formulasi Krim Ekstrak Etanol Daun Kirinyuh (Euphatorium odoratum. L) untuk Penyembuhan Luka. Artikel PharmaMedika, 3(1), 227–230. https://doi.org/10.33476/MKP.V3I1.440
[6] Barku, V. Y. A., Boye, A., & Ayaba, S. (2013). Phytochemical Screening and Assessment of Wound Healing Activity of the Leaves of Anogeissus leiocarpus. European Journal of Experimental Biology, 3(4), 18–25.
[7] Wirasti, W., Lestari, T., & Isyti’aroh, I. (2021). Penghambatan Ekstrak Daun Kremah (Alternanthera sessilis) terhadap Enzim α-Amilase secara In-Vitro. Pharmacon: Jurnal Farmasi Indonesia, 18(1), 68–74.
[8] Sugiwati, S., Setiasih, S., & Afifah, E. (2009). Antihyperglycemic Activity of the Mahkota Dewa Leaf Extracts as an Alpha-Glucosidase Inhibitor. Makara Journal of Health Research, 13(2), 74–78.
[9] Wicaksono, I. B., & Ulfah, M. (2017). Uji Aktivitas Antioksidan Kombinasi Ekstrak Etanol Daun Sirsak (Annona muricata L.) dan Daun Jambu Biji (Psidium guajava L.) dengan Metode DPPH (2,2-Difenil-1- Pikrilhidrazil). Inovasi Teknik Kimia, 2(1), 44–48.
[10] Khairina, A., & Leny, Y. (2015). Pengaruh Variasi Lama Penyimpanan Umbi Bengkuang (Pachirhyzus erozus) terhadap Kadar Glukosa Darah Rattus norvegicus. Unesa, Journal of Chemistry, 4(1), 31–36.
[11] Afriyanti, T. (2015). Uji Potensi Antidiabetik pada Ekstrak Daun Garcinia mangostana L. Melalui Penghambatan Aktivitas Alfa-Glukosidase dan Alfa-Amilase serta Penapisan Fitokimia pada Ekstrak Teraktif. Universitas Indonesia.
[12] Puiyanto, S., Wijanarka, Budi, R., & Via, A. (2019). Aktivitas Inhibitor α-Amilase Ekstrak Etanol Tanaman Brotowali (Tinospora crispa L.). Bioma, 21(2), 91–99.
[13] Meila, O., & Noraini. (2017). Uji Aktivitas Antidiabetes dari Ekstrak Metanol Buah Kiwi (Actinidia deliciosa) melalui Penghambatan Aktivitas α-Glukosidase. Jurnal Farmasi Galenika, 3(2), 132–137.
[14] Pambudi, B. D., Fajriyah, N. N., & Maharisti, R. A. (2021). Uji Aktivitas Penghambatan α-Amilase dalam Ekstrak Etanol Daun Adas (Foeniculum vulgare Mill.) Menggunakan Elisa Reader. Urecol Journal, Part C: Health Sciences, 1(1), 1–7.
[15] Tahapary, R. (2017). Identifikasi dan Penentuan Jenis Penghambatan Senyawa Anti-Amilase Asal Ekstrak Kulit Batang Gayam (Inocarpus edulis). Universitas Pattimura.
[16] Mugiyanto, E., Simanjuntak, P., & Setyahadi, S. (2017). Identifikasi Senyawa Aktif Fraksi Etanol Daun Sirsak (Annona muricata Linn.) sebagai Inhibitor α-Amilase. Journal Para Pemikir, 6(2), 131–138.
[17] Fadia, N., Herlina, T. E., & Lutpiatina, L. (2020). Efektivitas Ekstrak Etanol Daun Kirinyuh (Chromolaena odorata) sebagai Antibakteri Salmonella Typhi dan Staphylococcus auerus. Jurnal Riset Kefarmasian Indonesia, 2(3), 158–168.
[18] Panjuantiningrum, F. (2010). Pengaruh Pemberian Buah Naga Merah (H. polyrhizus) terhadap Kadar Glukosa Darah Tikus Putih yang Diinduksi Aloksan. Universitas Sebelas Maret.
[19] Tadera, K., Minami, Y., Takamatsu, K., & Matsuoka, T. (2005). Inhibition of α-Glukosidase and α-Amylase of Flavonoids. J Nutr. Sci Vitaminol, 52(2), 149–153.
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