Pengujian Toksisitas dan Antioksidan Ekstrak Etilasetat dan n-Butanol Hipokotil Sarang Semut, Myrmecodia pendens Merr. & Perry

  • Vino Soaduon Hamonangan Simanjuntak Vino Soaduon Hamonangan Simanjuntak
  • Endah Wydiastuti Fakultas Farmasi, Universitas Pancasila
  • Lina Marlina Pusat Riset Bahan Baku Obat dan Obat Tradisional (BBO OT), Badan Riset dan Inovasi Nasional (BRIN)
  • Partomouan Simanjuntak Pusat Riset Bahan Baku Obat dan Obat Tradisional (BBO OT), Badan Riset dan Inovasi Nasional (BRIN)
  • Rudi Kartika Jurusan Kimia, FMIPA, Universitas Mulawarman
DOI: https://doi.org/10.30872/jkm.v21i1.1288

Abstract

Sarang semut, Myrmecodia pendens Merr. & Perry (Rubiaceae) is an epiphytic plant from Papua which is used to cure various diseases. The purpose of this study is to see the chromatogram profile [Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC)] analysis, and biological test of ethyl acetate and n-butanol of Sarang semut’s hypocotyl extracts from methanol extracts. Meanwhile, the biological tests carried out were toxicity tests on Artemia salina L shrimp larvae and antioxidant assay with free radical scavenging methods (DPPH). The results of the antioxidant activity test showed that the antioxidant activity of the ethyl acetate extract was higher (IC50=18.23μg/ml) than the n-butanol extract (IC50=29.58μg/ml). The results of the toxicity test on the ethyl acetate extract were higher (LC50=101.84 g/ml) than the n-butanol extract (LC50=154.59 g/ml). TLC analysis results showed that the mobile phase for the best separation of chemical compounds in ethyl acetate extract was chloroform-methanol (10:1) and n-butanol extract was the mobile phase chloroform-methanol-water (5:5:1), while HPLC analysis showed that the mobile phase for the separation of chemical compounds in the ethyl acetate extract was n-hexane-ethyl acetate (10:1) and the n-butanol extract was methanol-water (10:1).

Keyword
Myrmecodia pendens- merr & perry, Rubiaceae, Antioxidant, Toxicities, HPLC, TLC

Downloads

Download data is not yet available.

References

[1] D. S. Fabricant and N. R. Farnsworth, “The value of plants used in traditional medicine for drug discovery.,” Environmental Health Perspectives, vol. 109, no. suppl 1, pp. 69–75, Mar. 2001, doi: 10.1289/ehp.01109s169.
[2] S. Duryatmo, “Sarang semut vs peyakit maut,” Majalah trubus, vol. 37, no. 439, pp. 12-15, 2006.
[3] A. Subroto, and H. Saputro, “Gempur penyakit dengan sarang semut,” Jakarta: Penebar Swadaya, hal. 26 – 28; 41-46, 2006.
[4] A. Soeksmanto, M. A. Subroto, H. Wijaya, and P. . Simanjuntak, “Anticancer Activity Test for Extracts of Sarang Semut Plant (Myrmecodya pendens) to HeLa and MCM-B2 Cells,” Pakistan Journal of Biological Sciences, vol. 13, no. 3, pp. 148–151, Jan. 2010, doi: 10.3923/pjbs.2010.148.151.
[5] P. Simanjuntak, Fanny, M. A. Subroto, “Isolasi Senyawa Aktif dari Ekstrak Hipokotil Sarang Semut (Myrmecodia pendens Merr. & Perry) sebagai Penghambat Xantinoksidase,” Jurnal Ilmu Kefarmasian Indonesia, vol. 8, no. 1, pp. 49-54, Apr. 30, 2010. [Online]. Available: http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/366
[6] Bustanussalam, M. Bintang, and P. Simanjuntak, “Molecular Structure Elucidation of the Polar Fraction ”Sarang semut” plant Myrmecodia pendens Merry & Perry, Which has Cytotoxic and as antioxidant” in International Conference on Biotechnology Proceeding, in Bogor, Indonesia, 13-14 Nov 2012, pp. 401 – 409, 2013
[7] Departemen Kesehatan Republik Indonesia. “Buku panduan teknologi ekstrak,” Jakarta: Direktorat Jendral Pengawasan Obat dan Makanan, hal. 10-14, 24, 2000.
[8] P. Molyneux, “The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant Activity,” Songklanakarin J. Sci. Technol, vol. 26, no. 2, pp. 211-219, 2004. [Online]. Available: https://www.researchgate.net/Philip-Molyneux-5/237620105
[9] B. N. Meyer, N. R. Ferrigni, J. E. Putnam, L. Jacobsen, D. E. Nichols, and J. L. McLaughlin, “Brine shrimp: a convenient general bioassay for active plant constituents,” Planta Medica, vol. 45, no. 05, pp. 31–34, May 1982, doi: 10.1055/s-2007-971236.
[10] Hanafi, C. Irawan, S. M. Sirait, L. Sulistiawaty, and S. R. Setyawati, “Toxicity Test with BSLT (Brine Shrimp Lethality Test) Method on Methanol, Ethyl Acetate Extract, Hexane on Seeds and Rind of Matoa extract (Pometia pinnata),” Oriental J. Chemistry, vol. 36, no. 6, pp. 1143–1147, Dec. 2020, doi: 10.13005/ojc/360618.
Published
2023-11-30
How to Cite
SIMANJUNTAK, Vino Soaduon Hamonangan et al. Pengujian Toksisitas dan Antioksidan Ekstrak Etilasetat dan n-Butanol Hipokotil Sarang Semut, Myrmecodia pendens Merr. & Perry. JURNAL KIMIA MULAWARMAN, [S.l.], v. 21, n. 1, p. 55-61, nov. 2023. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/1288>. Date accessed: 29 apr. 2024. doi: https://doi.org/10.30872/jkm.v21i1.1288.
Citation Formats
Issue
Vol 21 No 1 (2023)
Section
Artikel

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

This license requires that reusers give credit to the creator. It allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, even for commercial purposes. If others remix, adapt, or build upon the material, they must license the modified material under identical terms.

  • BY: Credit must be given to you, the creator.
  • SA: Adaptations must be shared under the same terms.

    •