Sintesis Askorbil Laurat Dari Metil Laurat Dan Asam Askorbat Melalui Reaksi Transesterifikasi Dengan Katalis Lipase Serta Uji Aktivitas Antioksidan
Antioxidant are defined as compound that is capable of delay, postpone or prevent oxidation reaction, such as on foods and drugs. Antioxidant can be found in nature but the used is relatively limited because hydrophilic. This research aims to modified natural antioxidant so as be used in lipophilic media. Methyl laurate is one of a fatty acid ester potentialy can be used in industry. Modified were made by synthesize of methyl laurate and ascorbic acid through transesterification with catalyst is lipase from sesame seeds (Sesamun indicum L.) in a organic solvent such as acetone. Transesterification process does on reacton time at 36 hours at temperature (40-50) ºC and pH is 7 so has yield produced is 53,89 %. The result is ascorbyl laurate identified of the functional groups using by FT-IR spectrophotometer. Based on the result identification of the functional groups by FT-IR spectrophotometer, then do the analysis of antioxidant activity with a Radical Reduction Method DPPH (2,2-diphenyl-1-picrylhidrazyl). Antioxidant activity value IC50 is 40,57 ppm. This result indicated the potential of ascorbyl laurate as antioxidant.
 Song Q.X., D.Z. Wei, W.Y Zhou, W.Q. Xu, S.L Yang. (2004). Enzymatic Synthesis and Antioxidant Properties of L-ascorbyl Oleate and L-ascorbyl Linoleate. Biotechnology Lett. 26: 1777-1780.
 Karmee S.K., (2009). Biocatalytic Synthesis of Ascorbyl Esters and Their Biotechnological Applications. Appl Microbiol Biotechnol. 81: 1013-1022.
 Lestari P. S., Harjono dan Supartono. (2012). Sintesis Askorbil Laurat Melalui Reaksi Esterifikasi Dengan Katalis Enzim Lipase. Semarang: Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Semarang.
 Arbianti, R., Utami, T. S., Hermansyah, H. dan Handayani, W. (2008). Pemanfaatan Biji Wijen Sebagai Sumber Enzim Lipase Untuk Reaksi Esterifikasi Gliserol-Asam Laurat Pada Pembuatan Agen Pengemulsi. Prosiding Semnas Rekayasa Kimia dan Proses. 1411-4216.
 Bradoo, S,, R., R.K. Saxena, R, Gupta. (1999). High yield of ascorbyl palmitate by thermostable lipase- mediated esterification. JAOCS. 76(11)
 Bouftira, I, Abdelly C. and Sfar S. (2007). Identification of a naturally occurring 2,6-bis (1,1-dimethylethyl)-4-methylphenol from purple leaves of the halophyte plant Mesembryanthemum crystallinum. African Journal of Biotechnology Vol. 6 (9), pp. 1136-1139. ISSN 1684–5315.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.