Isolasi Dan Karakterisasi Lipase Dari Rebung Bambu Betung Dendrocalamusasper

  • Agung Prasetyo Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
  • Winni Astuti Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman
  • Chairul Saleh Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Abstract

This research was conducted to get the extract of rough lipase produced by bamboo shoots (Dendrocalamus asper). Lipase isolation from bamboo shoots was done by blending bamboo shoots to produce the crude extract of the enzyme. Lipase crude extracts that occurred at centrifugation at 12000 rpm for 30 minutes at 4°C. The crude extracts obtained were then tested using quantitative pH 7, temperature 30ºC and substrate 1% (v/v). Based on research there is activity of crude extract of lipase equal to 2,24 U/ml.

Keyword
Lipase, Dendrocalamus asper, Specific Activity of Lipase

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References

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Published
2017-11-29
How to Cite
PRASETYO, Agung; ASTUTI, Winni; SALEH, Chairul. Isolasi Dan Karakterisasi Lipase Dari Rebung Bambu Betung Dendrocalamusasper. JURNAL KIMIA MULAWARMAN, [S.l.], v. 15, n. 1, p. 66-68, nov. 2017. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/585>. Date accessed: 17 dec. 2018. doi: https://doi.org/10.30872/jkm.v15i1.585.
Section
Artikel