ACTIVITIES OF AMYLASE, PROTEASE and LIPASE FROM HONEY Trigona sp, Apis mellifera and Apis dorsata
Abstract
Activities of amylase, protease and lipase from honey Trigona sp, Apis mellifera and Apis dorsata, determination protein concentration and the activity protease done with Bradford method, the determination of the glucose standard and activity amylase done with 3,5-dinitro salicylic acid (DNS) method and activity lipase done with acid-base titration with coconut oil substrate. The honey from Trigona sp has value of the activity amylase and lipase respectively by 0,0136 U / mg and 0,359 U/mg, whereas honey Apis mellifera has activity of protease, amylase and lipase of each 1,22 x 10-6 U/mg; 0,944 U/mg and 0,304 U/mg and then honey Apis dorsata has amylase and lipase activity of each of 0,0645 U/mg and 0,287 U/mg.
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References
[2] Badan Standarisasi Nasional Indonesia. 01-3545-1994.
[3] Sarwono B. 2001. Kiat Mengatasi Permasalahan Praktis Lebah Madu. Cetakan Pertama. PT.Agro Media Pustaka, Jakarta.
[4] Nadhilla N.F. 2014.”The Activity Of Antibacterial Agent Of Honey Against Staphylococcus aureus”. Journal majority, Volume 3 No.7.
[5] Surmalin L.O, Anna M., Prita W., Masitoh. 2014. “Aktivitas Antikanker dan Antioksidan Madu di Pasaran Lokal Indonesia”. Tesis, Program Studi Kimia, Universitas Islam Negeri Syariff Hidayatullah, Jakarta.
[6] Widyawati, Nurhaedar J., Citrakesumasari. 2013. “Pengaruh Pemberian Madu Terhadap Gula Darah Puasa Pasien Diabetes Melitus Tipe 2 Dengan Terapi Glibenklamid atau Metformin”. Tesis, program pasca sarjana Universitas Hasanudin, Makassar.
[7] Mohsen. 2009. “Effects of Natural Honey Comsumption in Diabetic Patients : an 8-week randomized clinical trial”. International Journal of Food Sciences and Nutrition; 60 (7): 618 – 626.
[8] Nadhilla N.F. 2014.”The Activity Of Antibacterial Agent Of Honey Against Staphylococcus aureus”. Journal majority, Volume 3 No.7.
[9] Suranto A. 2004. Khasiat dan Manfaat Madu Herbal. Agromedia Pustaka, Jakarta.
[10] Pavord, A.V. 1975. Bees and Beekeping. Redwood Burn Limited. London
[11] Gojmerac, W, L., 1983. Bee,Beekeping honey And Pollonation. Avi Publishing Company. Inc Westport Connecticut.
[12] Gunawan D. 2004. Ilmu Obat Alam Jilid I. Penebar Swadaya, Jakarta.
[13] Krell, R. 1996. “Value Added Products from Beekeeping.” FAO Agricultural Services Bulletin. No. 124. Food and Agriculture Organization of the United Nations Rome.
[14] Kuntadi.2008.“Perkembangan Koloni Apis Mellifera L. Yang Diberi Tiga Formula Kedelai Sebagai Pakan Buatan Pengganti Serbuksari” Jurnal Penelitian Hutan dan Konservasi Alam.5(4):367-397.
[15] Mahani, R.A. Karim dan N. Nurjanah. 2011. Keajaiban Propolis. Pustaka Bunda. Jakarta
[16] David. 2008. A Practical Manual of Beekeeping. Spring Hill: United Kingdom
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