ACTIVITIES OF AMYLASE, PROTEASE and LIPASE FROM HONEY Trigona sp, Apis mellifera and Apis dorsata

  • Hendric Surya Putra
  • Winni Astuti
  • Rudi Kartika

Abstract

Activities of amylase, protease and lipase from honey Trigona sp, Apis mellifera and Apis dorsata, determination protein concentration and the activity protease done with Bradford method, the determination of the glucose standard and activity amylase done with 3,5-dinitro salicylic acid (DNS) method and activity lipase done with acid-base titration with coconut oil substrate. The honey from Trigona sp has value of the activity amylase and lipase respectively by 0,0136 U / mg and 0,359 U/mg, whereas honey Apis mellifera has activity of protease, amylase and lipase of each 1,22 x 10-6 U/mg; 0,944 U/mg and 0,304 U/mg and then honey Apis dorsata has amylase and lipase activity of each of 0,0645 U/mg and 0,287 U/mg.

Keyword
Amylase, protease, lipase, bradford, DNS (3,5-dinitro salicylic acid), honey Trigona sp, Apis mellifera and Apis dorsata.

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Published
2018-11-29
How to Cite
PUTRA, Hendric Surya; ASTUTI, Winni; KARTIKA, Rudi. ACTIVITIES OF AMYLASE, PROTEASE and LIPASE FROM HONEY Trigona sp, Apis mellifera and Apis dorsata. JURNAL KIMIA MULAWARMAN, [S.l.], v. 16, n. 1, p. 27-31, nov. 2018. ISSN 2476-9258. Available at: <http://jurnal.kimia.fmipa.unmul.ac.id/index.php/JKM/article/view/433>. Date accessed: 20 sep. 2019. doi: https://doi.org/10.30872/jkm.v16i1.433.
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Artikel